Thursday, April 24, 2025

CLOSING THE GAP: HOW COMMUNITY-LED SANITATION CAN SOLVE THE GLOBAL WASTEWATER CRISIS

 

Author : AM.Tris Hardyanto

Introduction

Global wastewater management remains deeply unequal, with over 80% of untreated wastewater originating from developing regions where marginalized communities are often excluded from basic sanitation infrastructure. article explores the structural challenges and transformative potential of decentralized and community-led approaches to sanitation. Drawing on global case studies and current research, the work is structured across six s addressing infrastructure inequity, spatial exclusion, decentralized system effectiveness, market bias in public-private partnerships, funding and policy misalignment, and participatory planning models.

The overarching narrative advocates for community empowerment, spatial equity, and inclusive governance as critical pathways to a more just and sustainable sanitation future. By highlighting both systemic failures and emerging solutions, the article offers an evidence-based roadmap for policymakers, practitioners, and advocates working to close the global sanitation gap.

 

1 Infrastructure Inequity – Wastewater for the Few

The flush of a toilet is silent in cities like São Paulo, Cape Town, and Berlin—but deafening in places where the very idea of a sewer system is a distant dream. In one part of the world, wastewater disappears in seconds through engineered networks buried beneath skyscrapers. In another, it pools in open drains, seeps into drinking water, and sickens the very people it was meant to leave behind. The contrast is not a question of capacity—it is a question of will, inclusion, and justice.

 As the global sanitation crisis persists, it becomes increasingly evident that the problem is not simply technological—it is infrastructural inequity. The  explores how some of the world's most successful decentralized and community-led systems—like those in Delhi, Kenya, and Bangladesh—are challenging the legacy of exclusion. It asks: What happens when the people most affected by poor sanitation are given the power to design and manage their solutions? The answers offer not just innovation but hope. 

1.1 Decentralized Solutions: The Case of Delhi

The Decentralized Wastewater Treatment Systems (DEWATS) in Delhi effectively address persistent sanitation challenges for underserved communities. By enabling local wastewater treatment, DEWATS reduces the burden on centralized plants and significantly mitigates pollution, thus improving hygiene conditions in areas lacking conventional services (Miklos et al., 2018). decentralized model not only resolves immediate environmental and health issues but also fosters community engagement as locals manage and maintain these systems. Such participation ensures that sanitation improvements genuinely reflect community-specific needs (Miklos et al., 2018). Collaborative efforts by India's Ministry of Urban Development and NGOs further exemplify the impact of these decentralized initiatives, promoting equitable access to safer water for marginalized populations (Miklos et al., 2018).

Delhi's successful DEWATS implementation underscores the global shift toward community-driven wastewater management, a trend also evident in Kenya. 

1.2 Community Empowerment in Kenya

Kenya's community bio-centers similarly emphasize local participation in wastewater treatment. Beyond mere sanitation, these facilities serve as communal hubs, encouraging active resident involvement in management and operations. The approach effectively improves sanitation outcomes and enhances public health (Blatz et al., 2019). The role of the Kenya Water and Sanitation Civil Society Network (KEWASNET) highlights the importance of grassroots initiatives, providing critical education on sustainable water management to local communities (Blatz et al., 2019). Consequently, these bio-centers cultivate accountability and ownership, reinforcing sustainable sanitation practices and addressing urgent public health challenges (Blatz et al., 2019).

Kenya's bio-centres demonstrate that decentralized wastewater treatment combined with community empowerment can significantly improve health and environmental conditions, a practice mirrored in urban areas such as Bangalore. 

1.3 Urban Innovations Bangalore's Response

In Bangalore, several wastewater treatment plants operated by the Bangalore Water Supply and Sewerage Board (BWSSB) effectively confront urban pollution. These plants meet the increasing demand for clean water while mitigating untreated wastewater's environmental impacts through advanced technologies that enhance operational efficiency and sustainability (Galan-Lopez et al., 2019). Bangalore's proactive stance illustrates the growing global emphasis on innovative, efficient wastewater solutions crucial in rapidly urbanizing environments (Galan-Lopez et al., 2019).

The importance of urban wastewater innovation extends beyond Bangalore, with similar initiatives appearing in smaller urban municipalities such as those in Bangladesh.

1.4 Fecal Sludge Management in Bangladesh

Bangladesh's Pourashava Fecal Sludge Management (FSM) programs specifically target sanitation improvements in smaller, often underserved municipalities. These initiatives emphasize localized solutions, prioritizing marginalized communities and fostering collaboration between local governments and international NGOs to ensure sustainable faecal sludge management (Glabbeke et al., 1999). Bangladesh's community-driven FSM approach echoes the decentralized strategies evident in Delhi and Kenya, further validating the necessity of local engagement in effective sanitation management (Glabbeke et al., 1999).

The integration of sanitation with broader water management initiatives, as demonstrated by Brazil's efforts, further expands the potential of decentralized systems. 

1.5 Integrated Management in São Paulo

São Paulo's Integrated Water Management System represents an innovative blend of wastewater treatment and stormwater management, significantly enhancing urban sustainability and resilience. Developed by the São Paulo State Basic Sanitation Company (SABESP), the framework maximizes water reuse while controlling pollution effectively, addressing climate change and urban infrastructure needs (Herout et al., 2021). Integrated management strategies like São Paulo's offer comprehensive solutions capable of addressing current and future water challenges, illustrating a viable pathway toward urban resilience (Herout et al., 2021).

Cape Town's strategic approaches similarly highlight the effectiveness of integrated water management. 

1.6 Water Resilience Strategies in Cape Town

Cape Town's Water Resilience Strategy emphasizes wastewater reuse in addressing strategic water scarcity challenges. Managed by the City of Cape Town's Water and Sanitation Department, the strategy ensures equitable sanitation access while significantly reducing water wastage through treated wastewater reuse in non-potable applications (Lutz et al., 2020). strategic approach highlights the importance of sustainability and community engagement, equipping urban areas with resilience to potential water crises intensified by climate change (Lutz et al., 2020).

Cape Town's proactive management complements community-led initiatives in cities like Freetown, Sierra Leone. 

1.7 Community-Led Initiatives in Freetown

Freetown's Community-Led Sanitation Program actively empowers residents to manage their sanitation needs collaboratively. The participatory model promotes direct citizen engagement in planning and management, supported by international NGOs and local government (Marku et al., 2024). The program demonstrates the effectiveness of community-derived sanitation solutions explicitly tailored to local contexts, significantly enhancing public health outcomes (Marku et al., 2024).

Similarly, community committees in the Philippines underscore the significance of localized participation. 

1.8 Local Committees and Accountability in the Philippines

Community WASH Committees in the Philippines strengthen local participation in wastewater management, enhancing accountability and responsiveness to community needs. The model, facilitated through collaboration between local governments and NGOs, significantly improves water quality and hygiene practices, highlighting the essential role of local governance and participatory processes in adequate sanitation (Saltus et al., 2018).

Large urban centres like México City illustrate that extensive wastewater networks also play critical roles in sustainable urban development. 

1.9 Comprehensive Urban Management in México City

México City's extensive wastewater treatment network, managed by the Sistema de Aguas de la Ciudad de México (SACMEX), exemplifies comprehensive urban water management. Integrating wastewater treatment into broader environmental strategies, these facilities significantly enhance public health and water quality by effectively managing urban runoff (Child et al., 2017). holistic approach addresses environmental and public health concerns, demonstrating urban centres' potential to secure future water availability sustainably (Child et al., 2017).

Germany's technological leadership further enriches global wastewater management discussions. 

1.10 Advanced Technologies in Germany

Germany emphasizes advanced wastewater treatment technologies focused on resource recovery, extracting valuable nutrients and energy. These practices highlight innovation, efficiency, and environmental protection, offering valuable insights to countries pursuing enhanced resilience in water resource management (Chatterjee et al., 2018). 

1.11  Toward Global Sanitation Equity

Global trends increasingly favour decentralized, community-driven approaches to wastewater management. Initiatives across Delhi, Kenya, Bangladesh, Brazil, Cape Town, Freetown, the Philippines, México City, and Germany underscore the effectiveness of these localized models. By prioritizing community engagement, integrating advanced technologies, and emphasizing sustainability, policymakers can significantly enhance sanitation access, fostering healthier, more resilient urban environments worldwide. 

The examples presented—from India's DEWATS to México City's comprehensive urban systems—demonstrate that adequate sanitation infrastructure does not follow a one-size-fits-all blueprint. What connects them is not scale or capital but an ethic: the belief that every person deserves clean, safe, and dignified sanitation—regardless of their income, geography, or status. Whether through community bio-centers in Kenya or resilience strategies in Cape Town, the message is clear: when local knowledge meets political will, transformation follows.

The path toward global sanitation equity is not paved by mega-projects alone but by placing communities at the centre of planning, regulation, and implementation. Wastewater should not be a mark of poverty or neglect—it should be an opportunity to build systems that restore dignity, protect public health, and sustain our environment. The challenge is vast, but so is the potential for change—if we choose to invest in people, not just pipes.

 

 2 The Geography of Exclusion

In the boardrooms of multinational water giants, sustainability reports highlight innovation, efficiency, and growth. However, thousands of miles away in rural sub-Saharan Africa or in the overcrowded edges of South Asia's megacities, wastewater still runs untreated through open drains. The disconnect is not just geographic—it is systemic. It reflects a world where clean water access and sanitation infrastructure are distributed not by need but by profit potential. 

While Veolia, SUEZ, and Xylem engineer water solutions for the world's wealthiest regions, over 80% of untreated wastewater in developing countries pollutes rivers, endangers health, and widens inequality.  uncovers how geographic and economic exclusion, driven by skewed infrastructure investments and market-led decisions, creates a two-tiered sanitation system—one for the connected and profitable, another for the forgotten and voiceless. 

2.1 Corporate Leaders in Global Water Management

Veolia Environnement S.A., headquartered in France, leads globally in environmental services with €45.35 billion in revenue in 2023. The company excels in water management, wastewater treatment, waste recovery, and energy optimization. Veolia integrates advanced technologies and sustainable practices, prioritizing environmental protection and resource efficiency (Miklos et al., 2018).

Similarly, SUEZ S.A., another French entity, reported €17 billion in 2023, specializing in advanced water recycling and treatment solutions. Tailored services for municipalities and industries reflect their commitment to sustainability and technological innovation (Blatz et al., 2019).

Xylem Inc. from the U.S., with revenues of around $8.6 billion in 2024, specializes in pumps, filtration, and analytics. Xylem's intelligent water solutions enhance efficiency and water quality for municipal, agricultural, and industrial clients (Galan-Lopez et al., 2019). 

2.2 Diverse Approaches to Sustainability

Ecolab Inc., achieving $14.7 billion in revenue in 2023, excels in reducing water consumption and enhancing quality through chemical treatments and innovative systems. Its global partnerships underscore commitments to sustainable water access (Glabbeke et al., 1999).

American Water Works Company generated $4.5 billion in 2023, delivering reliable water and wastewater services across 46 U.S. states. Their infrastructure focus ensures that essential water utility operations are efficient and sustainable (Herout et al., 2021).

A.O. Smith Corporation, with $3.5 billion in revenue in 2023, specializes in water heating and treatment, significantly advancing consumer-focused water purification technologies (Lutz et al., 2020). 

2.3 Technological Innovations and Global Reach

Pentair plc, reporting $4.0 billion in revenue in 2023, delivers water filtration solutions across residential, industrial, and commercial markets. Their adaptable products highlight the critical role of customized water management in environmental stewardship (Marku et al., 2024).

Dow Inc., earning $56.5 billion in 2023, provides specialized wastewater treatment products such as advanced filtration membranes. Dow integrates sustainability into its offerings, ensuring regulatory compliance and environmental protection (Saltus et al., 2018).

Linde plc, operating internationally, generated $33.2 billion in 2023, offering water treatment solutions that aid industrial and municipal applications in meeting environmental standards and reducing consumption (Child et al., 2017).

Thermax Limited from India, with $1.2 billion in revenue, provides tailored industrial wastewater treatments, effectively managing environmental impacts through customized solutions (Chatterjee et al., 2018). 

2.4 Addressing Global Inequities

Despite advancements by these corporations, significant disparities persist, especially in developing nations. Over 80% of untreated wastewater originates from developing countries, highlighting the systemic exclusion of informal and rural communities in infrastructure planning (Giwa et al., 2024). Such inequities amplify health risks and deepen socioeconomic divides, underscoring a crucial need for equitable infrastructure investment (Afferden et al., 2015; Starkl et al., 2022).

Inadequate wastewater systems expose marginalized communities to significant health threats, exacerbating structural inequalities. Research highlights the urgent requirement for targeted interventions to alleviate these disparities and promote environmental justice (Kazora & Mourad, 2018). 

2.5 Inclusive Governance and Strategic Interventions

Prioritizing marginalized communities through inclusive governance and strategic infrastructure investments can significantly mitigate these inequities. Data-driven policies, alongside collaboration between corporations, governments, and NGOs, can effectively direct resources to underserved regions (Rüd & Marth, 2011).

Corporations like Veolia, SUEZ, and Dow could substantially reduce global sanitation inequities by leveraging their resources to expand services into neglected communities. Collaborative frameworks and inclusive planning are essential to bridging the gap and ensuring universal sanitation access (Kazora & Mourad, 2018). 

2.6  Equitable Water Management

The geography of exclusion reveals that equitable access to wastewater treatment is vital for global public health and environmental sustainability. Industry leaders, policymakers, and communities must collaboratively prioritize inclusive, innovative, and sustainable solutions. By addressing infrastructural disparities through targeted investments and comprehensive policies, universal access to sanitation can be realized, fostering healthier and more equitable communities worldwide.

Rivers and cities do not just shape the global map of wastewater infrastructure—it is carved by power, profit, and neglect. While major corporations pioneer cutting-edge technologies, billions continue to live without even the most basic sanitation, trapped in a geography of exclusion reinforced by policy inertia and market bias. However,  reality is not inevitable.

By redirecting innovation toward equity and aligning investment with the needs of marginalized populations,  geography can be redrawn. It begins with inclusive governance, data-driven planning, and corporate accountability. When companies, governments, and communities collaborate across these lines of division, wastewater systems become more than pipes and plants—they become instruments of justice. It is time we re-engineered not just our infrastructure but our priorities.

 

 3 Decentralized Systems – Promise or Problem?

When centralized infrastructure stops at the city's edge, who answers the call for sanitation? In many parts of the world—from Nairobi's informal settlements to remote corners of India and Bangladesh—the answer lies in decentralized wastewater systems. These systems represent more than pipes and tanks; they symbolize the potential of locally governed solutions to address deeply rooted gaps in public health and dignity. However, behind every successful community-run facility are dozens more that falter—not for lack of need, but for lack of support.

Decentralized systems have emerged as the pragmatic bridge between ambition and accessibility. They offer the rare promise of adaptability, affordability, and community ownership. However, as the  reveals, without regulation, investment, and inclusive planning, that promise is often broken. The question is not whether decentralized systems work—but whether we are willing to make them work for the people who need them most. 

3.1 Potential and Pitfalls of Decentralized Systems

Decentralized wastewater systems promise substantial benefits for improving sanitation access, particularly in underserved areas. Examples from India's DEWATS and Kenya's community bio-centers demonstrate effectiveness when adequately supported by governments and NGOs (Warshauer, 2018; Agrahari & Kumar, 2023). However, without sufficient funding, regulation, and maintenance, these systems frequently fail, undermining their intended benefits and potentially creating new health risks for communities (Rao, 2025). 

3.2 Community Management and Sustainability

Successful decentralized models emphasize local community management, fostering more substantial community ownership and greater sustainability. Community-led systems often achieve higher operational standards due to increased accountability and engagement (Edunjobi, 2024). However, for these systems to realize their full potential, consistent financial backing and robust governance frameworks are essential. These supportive structures provide the necessary resources and training for communities, ensuring ongoing operational effectiveness and sustainability (Posnaya et al., 2024). 

3.3 Governance and Financial Integration

A critical issue is the frequent mismatch between the potential of decentralized systems and their practical execution. Effective governance and financial mechanisms must be integrated from inception to implementation. Governments and development agencies must strategically invest in regulatory frameworks and capacity-building initiatives, aligning financial resources with governance needs to maintain high-quality standards and prevent system failures (Narasimhan et al., 2023). 

3.4 Policy Shifts and Strategic Investments

Realizing the promise of decentralized wastewater solutions requires policy shifts that prioritize these systems, not only in infrastructure but also in governance and community capacity-building. Policies must facilitate targeted investments in training, regulatory oversight, and community support mechanisms, transforming decentralized wastewater management from an idealistic concept to a practical, impactful reality (Ghawi, 2018). 

3.5 The Future of Decentralized Wastewater Management

Decentralized wastewater systems are poised to become integral to global sanitation strategies, especially in regions where centralized infrastructure is impractical or prohibitively expensive. However, their success is contingent upon targeted financial investments, policy reforms, and robust partnerships between communities, governments, and international organizations (Mujahidah et al., 2024). Strategic investments in governance structures and community capacity building will be crucial for scaling decentralized systems sustainably. 

3.6  From Potential to Practical Execution

Decentralized wastewater systems offer significant promise for improving sanitation, particularly in marginalized regions. However, their effectiveness relies heavily on strong governance, targeted investments, and active community engagement. By addressing governance and funding challenges, policymakers and stakeholders can bridge the gap between the theoretical advantages and practical execution of decentralized sanitation solutions, ultimately enhancing public health and promoting environmental sustainability worldwide.

The future of wastewater management will not be built solely in boardrooms or ministerial corridors—it will be shaped in village meetings, neighbourhood committees, and decentralized treatment hubs tucked behind community centres. The global sanitation crisis demands solutions that are nimble, inclusive, and scalable. Decentralized systems tick all those boxes—but only if they are treated not as afterthoughts but as frontline infrastructure.

To move from promise to performance, we must invest as much in the people running these systems as we do in the technologies themselves. Governance must be localized. Finance must be fair. Training must be continuous. In the end, a decentralized system that fails is not just a missed opportunity—it is a betrayal of the very communities it was designed to serve. The next  in sanitation equity will be written not in concrete terms but in trust. 

 4  PPPs and Market Bias

Public-private partnerships (PPPs) were once heralded as the silver bullet for solving infrastructure gaps. However, when it comes to sanitation, these partnerships often tell a different story—one of exclusion, skewed priorities, and market-driven neglect. In city centres and high-income enclaves, PPPs have delivered high-end wastewater treatment systems. In informal settlements and remote villages, however, they have delivered little more than silence.

The promise of PPPs lies in their ability to pool resources and expertise across sectors. However, when profitability eclipses public interest, sanitation becomes a service for the highest bidder—not the communities who need it most.  unpacks how PPP frameworks, if left unchecked, entrench geographic and economic inequalities in sanitation access—and what must change for these partnerships to serve the public good truly. 

4.1 Market Bias and Inequitable Outcomes

Public-private partnerships (PPPs) frequently demonstrate a bias toward affluent industrial or urban areas, sidelining marginalized communities from essential wastewater treatment services. Investments emphasizing short-term economic returns often result in inequitable sanitation systems, disproportionately exposing impoverished communities to untreated wastewater and associated health risks (Adebayo et al., 2024; Kazora & Mourad, 2018). systematic bias perpetuates social inequalities, leading to significant public health disparities. 

4.2 The Impact of Skewed Investments

The prioritization of profitability within PPP frameworks perpetuates exclusion, leaving vulnerable populations without access to clean and safe sanitation services. Oversight traps marginalized communities in cycles of poor health, environmental degradation, and social exclusion. The failure to achieve spatial inclusion further exacerbates disparities, disproportionately impacting regions most vulnerable to waterborne diseases (Gómez-Román et al., 2021; Naik & Stenstrom, 2016). 

4.3 Challenges in the Implementation of The Bangladesh Case

Bangladesh's pourashava faecal sludge management (FSM) programs illustrate both the potential and pitfalls of current PPP arrangements. Despite intended equity-focused initiatives, inconsistent funding and weak regulatory oversight frequently undermine these programs. Consequently, marginalized communities continue to suffer from inadequate sanitation solutions, highlighting the critical need for consistent support and robust governance frameworks (Khoroshilov, 2020; Capodaglio, 2017). 

4.4 Integrating Pro-Poor Objectives

Addressing systemic biases within PPPs requires embedding affordability, accountability, and spatial inclusivity within their operational frameworks. Policymakers and private sector participants must shift their focus from short-term gains to long-term inclusive sustainability. Strategic alignment with pro-poor objectives is vital for reducing inequalities and improving sanitation access for marginalized communities (Kwiringira et al., 2021; Ali & Khan, 2023). 

4.5 Policy Reforms and Strategic Alignment

Essential reforms in regulatory frameworks and policy adjustments are necessary to ensure equitable sanitation service delivery. Integrating pro-poor objectives within PPP models is crucial for achieving sustainable outcomes and enhancing public health across all societal sectors. Strategic policy reforms should prioritize investments that support governance, community engagement, and long-term operational sustainability (Bahman & Yazdian, 2024; Shukla et al., 2021). 

4.6 Enhancing Community Capacity

Successful decentralized sanitation models highlight the importance of local community engagement and capacity-building. Investing in training, infrastructure, and robust governance frameworks is essential for empowering communities to manage their sanitation services sustainably. The community-driven approach ensures the adaptability and relevance of sanitation solutions, addressing the unique needs of underserved regions (Igoud et al., 2020; Bailey et al., 2020). 

4.7 Technological Innovations and Inclusive Policies

Advances in decentralized wastewater technologies, including microfiltration and ultraviolet disinfection, offer significant potential for improving localized sanitation. However, their success hinges on inclusive policies that accommodate the socioeconomic contexts of marginalized populations. Effective integration of these technologies requires comprehensive planning, policy support, and targeted resource allocation (Xue, 2022; Khattiyavong & Lee, 2019). 

4.8  Toward Inclusive PPPs

Addressing inherent biases within PPPs demands transformative change toward equitable sanitation access. By recalibrating PPP frameworks to prioritize marginalized communities, stakeholders can cultivate resilient and inclusive sanitation systems. Strong collaboration between local governments, communities, and the private sector is essential for achieving equitable sanitation outcomes, ultimately fostering healthier and more inclusive societies (Kuttuva et al., 2018).

The failure of PPPs to deliver equitable sanitation is not a failure of collaboration—it is a failure of vision. When market logic dominates public service, the most vulnerable are left with empty promises and untreated waste. However, it can change. Pro-poor policy reforms, inclusive financing, and strong regulatory guardrails can reorient PPPs toward justice rather than just returns.

True partnership means shared responsibility, shared accountability, and shared outcomes. Governments must demand more. Communities must be part of the design. Moreover, the private sector must recognize that the future of sanitation is not in the margins—it is in bridging the margins. Only by recalibrating these partnerships with equity at the core can we transform PPPs from instruments of division into engines of inclusive development. 

 5 Funding and Policy Misalignment

In a world overflowing with climate pledges, development loans, and sustainability funds, why do the poorest still lack toilets? The answer lies not in scarcity—but in skewed priorities. Sanitation financing today remains fixated mainly on high-visibility urban projects, leaving behind the informal settlements, peri-urban peripheries, and rural heartlands that need it most. The result? A landscape of gleaming treatment plants in capital cities and open defecation in forgotten communities. 

Funding shapes futures. Moreover, when those funds ignore the geographies and histories of exclusion, they reinforce them.  examines the profound misalignment between where sanitation resources are most needed and where they actually go. It argues for a new financial logic—one grounded not in GDP or project return but in rights, justice, and equity. 

5.1 Misalignment of Funding Priorities

Funding for sanitation often neglects underserved communities, favouring large-scale infrastructure projects primarily in urban areas. Climate adaptation financing and SDG-aligned funds frequently bypass informal settlements and rural regions, exacerbating social inequities and hindering progress towards universal sanitation (Aras & Furtuna, 2024; Hyde‐Smith et al., 2024). Critical analysis of these funding distributions reveals structural barriers that perpetuate exclusion, demanding a comprehensive reevaluation of current funding models. 

5.2 Rights-Based Approaches to Sanitation

Adopting a rights-based approach could significantly rectify existing funding imbalances by recognizing sanitation as a fundamental human right. Such an approach ensures equitable access across geographic and socioeconomic divides and can be effectively supported through targeted subsidies. Policies at both international and national levels must prioritize marginalized groups, enforcing strict adherence to equitable resource distribution to prevent fund diversion towards higher-return projects (Fatajo & Barrow, 2023; Hyde‐Smith et al., 2024). 

5.3 Leveraging Geographic Insights WASH Equity Atlases

The development of WASH Equity Atlases provides valuable geographic insights into infrastructure disparities. Despite advancement, the full potential of these tools remains unrealized in policymaking. Further exploration into how policymakers utilize geographic data can help address practical barriers to equitable resource allocation. Enhanced integration of these insights into decision-making processes is crucial for effective sanitation strategies and improved accountability (Sami et al., 2024). 

5.4 Spatial Equity in Funding Models

Incorporating spatial equity into sanitation funding models necessitates a deeper understanding of the social dynamics and historical contexts of communities. Current mechanisms must evolve to include not only infrastructure needs but also socioeconomic factors such as income disparities and historical exclusion. Investigating these dynamics will inform the development of holistic funding strategies aimed at addressing underlying causes of inequality and ensuring equitable resource allocation (Wallock et al., 2024).

5.5 Sustainability and Scalability of Equity-Based Models

Evaluating the long-term sustainability of equity-based funding models is essential to ascertain their viability and scalability. Case studies where spatial equity has been effectively implemented can offer insights into the factors contributing to success. Exploring evidence that demonstrates improved outcomes from equity-aligned funding strategies will help validate their adoption as a sustainable approach to sanitation management (Li et al., 2021). 

5.6  Towards Equitable Sanitation Financing

Addressing funding and policy misalignment requires more than structural adjustments; it demands a commitment to social justice and equity. By critically examining funding allocations, rights-based approaches, geographic tools, spatial equity, and sustainability, policymakers and stakeholders can foster more inclusive and equitable sanitation landscapes. A comprehensive approach ensures that vulnerable communities receive the necessary attention and resources, ultimately achieving sustainable sanitation solutions that benefit all populations.

Funding is never neutral. It reflects who we value, what we prioritize, and how we define progress. When sanitation investments bypass the most vulnerable, they do not just miss the mark—they perpetuate harm. However, change is possible. Rights-based approaches, spatial equity tools like WASH Equity Atlases, and inclusive policymaking offer a blueprint for realignment.

The challenge ahead is not just about fixing pipes or building facilities. It is about rewriting the financial DNA of sanitation policy to ensure that no one is left behind—not because it is charitable, but because it is just. Because when funding follows equity, sanitation becomes not a privilege for the few but a public good for all. 

 6 Community-Led Infrastructure Planning

What happens when the people who need sanitation the most are finally invited to design it? Across Nigeria, Zambia, and the Philippines, a quiet revolution is taking shape—led not by engineers or ministers but by mothers, teachers, farmers, and youth. These are the architects of a new sanitation movement: one rooted in community, guided by lived experience, and built to last. Community-led infrastructure planning is not a footnote in the sanitation debate—it is the frontline. 

For too long, sanitation solutions have been imposed on communities rather than built with them. The result? Systems that fail to reflect local realities are poorly maintained and, ultimately, abandoned.  explores how participatory planning—when done authentically—does more than deliver infrastructure. It builds resilience, unlocks ownership, and restores dignity to the people long excluded from decisions about their fundamental rights. 

6.1 The Importance of Participatory Planning

Participatory infrastructure planning emerges as a critical strategy for enhancing wastewater management and addressing sanitation inequity. Community-driven models, such as WASH committees in Nigeria and the Philippines, significantly improve both the adoption and longevity of sanitation solutions (Zhang, 2024). These models foster local accountability, ensuring communities maintain ownership and responsibility for sanitation outcomes, thereby enhancing resilience and effectiveness. 

6.2 Fostering Local Ownership and Accountability

Local ownership generated through participatory planning leads to heightened accountability among residents. Community-led management fosters a greater investment in infrastructure maintenance and sustainability, thus transforming sanitation services from externally imposed obligations into community-driven initiatives. Collective responsibility promotes social cohesion and community pride, which are essential for long-term sustainability (Tomberge et al., 2021). 

6.3 Aligning Sanitation Solutions with Cultural Contexts

Cultural alignment is integral to the success of community-led planning initiatives. Unlike top-down approaches, community-led projects incorporate local cultural values and social norms from inception, enhancing acceptance and technical feasibility (Turrén-Cruz et al., 2020). Such contextually sensitive planning ensures that sanitation interventions align closely with community expectations and preferences, improving their sustainability and effectiveness. 

6.4 Empowerment Through Capacity Building

Capacity-building and training programs play pivotal roles in empowering communities to actively participate in managing sanitation infrastructure. By equipping residents with essential skills and knowledge, these initiatives foster self-reliance and reduce dependency on external support. Empowerment facilitates continuous learning and adaptability, enabling communities to independently maintain sanitation systems effectively (Venkataramanan et al., 2018). 

6.5 Inclusive Governance and Community Resilience 

Inclusive governance is crucial to the long-term success of community-led infrastructure planning. Ensuring that marginalized groups—particularly women, youth, and vulnerable populations—actively participate in decision-making processes strengthens community resilience and promotes equitable sanitation outcomes (Ficek & Novotný, 2018). For example, in Zambia, inclusive community engagement in the planning and implementation of sanitation initiatives resulted in significantly increased latrine coverage and improved hygiene practices, especially among women and children (Yeboah‐Antwi et al., 2019). Similarly, in Nepal, involving women's groups in the governance of local WASH projects led to higher project completion rates and more sustained infrastructure maintenance, illustrating the practical impact of inclusive participation on sanitation success. An inclusive approach enhances social justice and ensures comprehensive infrastructure that meets diverse community needs. 

6.6 Practical Applications Case Studies from Nigeria and the Philippines

Examples from Nigeria and the Philippines demonstrate the tangible benefits of community-led infrastructure planning. In these contexts, community WASH committees have effectively increased sanitation access and improved system management through active community engagement. These cases underline the practical advantages of participatory models, showcasing enhanced health outcomes and sustainable sanitation practices (Zhang, 2024). 

6.7  Strengthening Communities Through Participation

Community-led infrastructure planning is not merely a technical solution; it embodies principles of empowerment, cultural sensitivity, and inclusivity essential for addressing sanitation inequalities. Policymakers and stakeholders should prioritize community engagement and capacity building, ensuring that sanitation solutions are responsive, sustainable, and socially equitable. By placing communities at the centre of sanitation planning, significant improvements in public health, social cohesion, and overall quality of life can be achieved for underserved populations. 

Community-led infrastructure planning is not a charitable add-on—it is a strategic necessity. When communities are treated as co-creators, not just beneficiaries, sanitation systems are more resilient, more sustainable, and more just. The evidence from Zambia, Nepal, and the Philippines is clear: participatory governance leads to better outcomes, deeper accountability, and stronger local capacity.

If policymakers and planners are serious about ending the global sanitation crisis, they must start by handing over the blueprint. Let communities lead. Let culture shape design. Let participation drive investment. Because the future of sanitation will not be engineered from above—it will rise from the ground up, where dignity, ownership, and justice begin.

  

Conclusion

Infrastructure inequity within wastewater systems remains a significant barrier to health, dignity, and environmental justice. Disproportionate burdens imposed on vulnerable populations—from Indigenous communities to urban slums—underscore the urgent need for equitable investments, decentralized innovations, and participatory governance. Strategies to remedy  injustice must include robust regulation of decentralized systems, pro-poor reforms in PPP frameworks, targeted funding for underserved regions, and community-led infrastructure planning initiatives.

Utilizing spatial equity tools and pursuing inclusive finance will help redefine sanitation from a privilege reserved for the few to a fundamental right for all. Only through deliberate and just actions can wastewater infrastructure serve the diverse communities it aims to protect, paving the way for a more equitable future. 

Infrastructure inequity within wastewater systems remains a persistent obstacle to health, dignity, and environmental justice across the globe. Disproportionate burdens imposed on vulnerable populations, including Indigenous communities and urban slum dwellers, accentuate the urgent necessity for equitable investments, decentralized innovations, and participatory governance mechanisms. The existing systems of sanitation create disparities that directly impact community well-being, calling for a multifaceted approach to remedy these injustices through several key strategies.

Firstly, robust regulation of decentralized systems is essential to ensure that they operate efficiently and sustainably while serving the needs of marginalized communities. Research indicates that community engagement in sanitation projects generates positive outcomes, ultimately leading to improvements in health and environmental conditions McGranahan (2015). However, such systems must be backed by regulatory frameworks that guarantee their effective operation.

Additionally, pro-poor reforms in public-private partnership (PPP) frameworks are crucial. These reforms should aim to redirect funding towards underserved regions, thereby addressing the historical neglect these areas have faced in sanitation investments (Tsinda et al., 2015). Targeting specifically low-income and marginalized communities can help to alleviate sanitation inequities that have persisted over decades. Only by engaging these communities in meaningful ways can we hope to foster the kind of social cohesion necessary for effective sanitation solutions.

Community-led infrastructure planning initiatives present another promising avenue for improving access to sanitation services. Through participatory processes, residents can take ownership of sanitation projects, ensuring interventions are not only culturally appropriate but also sustainable in the long term (Pan et al., 2016). ownership fosters local accountability, increasing the likelihood that these systems will be maintained and utilized effectively.

Utilizing spatial equity tools will help in understanding the disparities within areas that require attention. By mapping infrastructure gaps and identifying the communities that lack adequate sanitation services, stakeholders can prioritize investments where they are most needed (Delaire et al., 2020). targeted funding approach enables better-aligned resource allocation strategies that can address systemic inequalities in access to sanitation.

Furthermore, pursuing inclusive finance approaches is essential in mitigating the inequities prevalent in sanitation systems. By recognizing sanitation as a fundamental right rather than a privilege, financing should be structured to alleviate barriers faced by the most vulnerable populations (Minh & Hung, 2011). may involve innovative financial mechanisms that facilitate access to sanitation services for underserved communities, ensuring that financing flows equitably.

Ultimately, addressing these structural inequities requires deliberate and just actions from all stakeholders involved. Policymakers and development agencies must work collaboratively with communities to develop responsive, equitable, and sustainable sanitation strategies that prioritize human rights and dignity for all individuals. By doing so, wastewater infrastructure can effectively serve the diverse communities it aims to protect, leading to a more equitable and just future.

The ongoing challenges of infrastructure inequity within wastewater systems necessitate a concerted effort to redefine sanitation from a privilege reserved for a select few to a fundamental right accessible to all. Through equitable investments, decentralized innovations, and participatory governance, we can create a more just and sustainable world where the right to safe sanitation is recognized and upheld universally.



 References

 (2020). Partnership aims to improve access to clean, safe and sustainable water in India. *Membrane Technology, 2020(12)*, 14-14. https://doi.org/10.1016/s0958-2118(20)30217-2

Aimé Tsinda, Pamela Abbott, Jonathan Chenoweth (2015). Sanitation markets in urban informal settlements of East Africa. *Habitat International, 49*, 21-29. https://doi.org/10.1016/j.habitatint.2015.05.005

Ali Hadi Ghawi (2018). Study on the Development of Household Wastewater Treatment Unit. *Journal of Ecological Engineering, 19(2)*, 63-71. https://doi.org/10.12911/22998993/81780

Amol Shukla, Divyesh Parde, Varun Gupta, Ritesh Vijay, Rakesh Kumar (2021). A review on effective design processes of constructed wetlands. *International Journal of Environmental Science and Technology, 19(12)*, 12749-12774. https://doi.org/10.1007/s13762-021-03549-y

Anamika Chatterjee, Christina Feldmann, Jasmin S. Hanke, Marcel Ricklefs, Malakh Shrestha, Guenes Dogan, Axel Haverich, Jan D. Schmitto (2018). The momentum of HeartMate 3: a novel active magnetically levitated centrifugal left ventricular assist device (LVAD). *Journal of Thoracic Disease, 10(S15)*, S1790-S1793. https://doi.org/10.21037/jtd.2017.10.124

Andrea G. Capodaglio (2017). Integrated, Decentralized Wastewater Management for Resource Recovery in Rural and Peri-Urban Areas. *Resources, 6(2)*, 22. https://doi.org/10.3390/resources6020022

Arun Kumar Sharma, Ashok Chopra (2013). Removal of COD and BOD from biologically treated municipal wastewater by electrochemical treatment. *Journal of Applied and Natural Science, 5(2)*, 475-481. https://doi.org/10.31018/jans.v5i2.356

Caroline Delaire, Rachel Peletz, Salim Haji, Joan Kones, Edinah Samuel, Alicea Easthope-Frazer, Eloïse Charreyron, Timothy C. Wang, Andy Feng, Razin Mustafiz, Ismat Jabeen Faria, Prince Antwi-Agyei, Emmanuel Donkor, Kwaku Amaning Adjei, Isaac Monney, Joyce Kisiangani, Clara MacLeod, Brian Mwangi, Ranjiv Khush (2020). How Much Will Safe Sanitation for all Cost? Evidence from Five Cities. *Environmental Science & Technology, 55(1)*, 767-777. https://doi.org/10.1021/acs.est.0c06348

Catherine W. Saltus, Brian Calingaert, Sean D. Candrilli, María Gabriela Lorenzo, Yulia Dyachkova, Thorsten Otto, Uwe Wagner, James A. Kaye (2018). Epidemiology of Adult Soft-Tissue Sarcomas in Germany. *Sarcoma, 2018*, 2025-11-01 00:00:00. https://doi.org/10.1155/2018/5671926

Chanthephar Khattiyavong, Han Soo Lee (2019). Performance Simulation and Assessment of an Appropriate Wastewater Treatment Technology in a Densely Populated Growing City in a Developing Country: A Case Study in Vientiane, Laos. *Water, 11(5)*, 1012. https://doi.org/10.3390/w11051012

Cristina Gómez-Román, José Manuel Sabucedo Cameselle, Mónica Álzate, Beatriz Medina (2021). Environmental Concern Priming and Social Acceptance of Sustainable Technologies: The Case of Decentralized Wastewater Treatment Systems. *Frontiers in Psychology, 12*, . https://doi.org/10.3389/fpsyg.2021.647406

David B. Miklos, Christian Remy, Martin Jekel, Karl G. Linden, Jörg E. Drewes, Uwe Hübner (2018). Evaluation of advanced oxidation processes for water and wastewater treatment – A critical review. *Water Research, 139*, 118-131. https://doi.org/10.1016/j.watres.2018.03.042

Diana Paola Bernal, Inés Restrepo, Simón Grueso-Casquete (2021). Key criteria for considering decentralization in municipal wastewater management. *Heliyon, 7(3)*, e06375. https://doi.org/10.1016/j.heliyon.2021.e06375

Dorjan Marku, Ardita Hoxha-Jahja, Ines Maurmann (2024). Impact of digital technologies on farm operational activities: A case study analysis between Germany and Finland. *nan, 1(1)*, 17-24. https://doi.org/10.33422/icrbmf.v1i1.519

Ebrima Fatajo, Mustapha Barrow (2023). Water, Sanitation, and Hygiene Policies and the Enhancement of Quality Education in Lower and Upper Basic Schools within Region One Education Directorate, The Gambia. *nan, 3(2)*, 14-25. https://doi.org/10.61090/aksujacog.2023.002

Elis Hastuti, Sri Darwati, Fitrijani Anggraini (2019). SAFE REUSE OF TREATED WASTEWATER AND SLUDGE IN DECENTRALIZED SYSTEM. *Matter International Journal of Science and Technology, 5(2)*, 175-189. https://doi.org/10.20319/mijst.2019.52.175189

Faisal Merghani Ibrahim, Bashir Mohammed El Hassan, Ahmed Elnadif Elmanssury, Ahmed Musa Siyam, Safa Abdaalla Dafaallah, Yasir Hayat Mughal, Mahmoud Jaber (2024). Integrated, Decentralized Wastewater Management Use to Improve the Environmental Health of Khartoum Locality Sudan. *nan, *, . https://doi.org/10.31219/osf.io/ezp95

František Ficek, Josef Novotný (2018). Comprehending practitioners’ assessments of community-led total sanitation. *Health Promotion International, 34(6)*, e129-e138. https://doi.org/10.1093/heapro/day070

Ghazaleh Bahman, Hamed Yazdian (2024). Developing a Water, Food, Energy, Economy, and Environment Nexus Index for Evaluating Centralized and Decentralized Wastewater Treatment Systems. *Environmental Quality Management, 34(2)*, . https://doi.org/10.1002/tqem.22344

Gordon McGranahan (2015). Realizing the Right to Sanitation in Deprived Urban Communities: Meeting the Challenges of Collective Action, Coproduction, Affordability, and Housing Tenure. *World Development, 68*, 242-253. https://doi.org/10.1016/j.worlddev.2014.12.008

Hoàng Văn Minh, Nguyen Viet Hung (2011). Economic Aspects of Sanitation in Developing Countries. *Environmental Health Insights, 5*, EHI.S8199. https://doi.org/10.4137/ehi.s8199

Hurriyah Mujahidah, Utomo Sarjono Putro, Khrisna Ariyanto (2024). Social Perception and Support of a Public Sanitation Service in Bandung: Community Insights and Engagement Strategies. *Journal of Social and Political Sciences, 7(2)*, . https://doi.org/10.31014/aior.1991.07.02.496

Jabir Ali, Waseem Khan (2023). Demographic, social and economic factors affecting the adoption of green toilets among rural households in India. *Environment Development and Sustainability, 26(2)*, 5117-5138. https://doi.org/10.1007/s10668-023-02927-5

Japheth Kwiringira, Robert Kabumbuli, Henry Zakumumpa, James Mugisha, Mathias Akugizibwe, Paulino Ariho, Joseph Rujumba (2021). Re-conceptualizing sustainable urban sanitation in Uganda: why the roots of ‘Slumification’ must be dealt with. *BMC Public Health, 21(1)*, . https://doi.org/10.1186/s12889-021-11029-8

Jonathan Roy Bailey, Sajjad Ahmad, Jacimaria R. Batista (2020). The Impact of Advanced Treatment Technologies on the Energy Use in Satellite Water Reuse Plants. *Water, 12(2)*, 366. https://doi.org/10.3390/w12020366

Julie Skrydstrup, Sille Lyster Larsen, Martin Rygaard (2020). Eco‐efficiency of water and wastewater management in food production: A case study from a large dairy in Denmark. *Journal of Industrial Ecology, 24(5)*, 1101-1112. https://doi.org/10.1111/jiec.13011

Kartiki S. Naik, Michael K. Stenstrom (2016). A Feasibility Analysis Methodology for Decentralized Wastewater Systems – Energy‐Efficiency and Cost. *Water Environment Research, 88(3)*, 201-209. https://doi.org/10.2175/106143016x14504669767337

Kojo Yeboah‐Antwi, William MacLeod, Godfrey Biemba, Patrick Sijenyi, Alexandra Höhne, Lavuun Verstraete, Caitryn McCallum, Davidson H. Hamer (2019). Improving Sanitation and Hygiene through Community-Led Total Sanitation: The Zambian Experience. *American Journal of Tropical Medicine and Hygiene, 100(4)*, 1005-1012. https://doi.org/10.4269/ajtmh.18-0632

Leonie Hyde‐Smith, Katy Roelich, Anna Mdee, Zhe Zhan, Barbara Evans (2024). Blinded by the ‘green-halo’? Equity in financing climate adaptation of urban sanitation. *Environment and Planning E Nature and Space, 8(1)*, 278-305. https://doi.org/10.1177/25148486241287446

Lidia Galan-Lopez, Jorge Barcia‐González, Eliseo Plasencia (2019). A systematic review of the accuracy and efficiency of dental movements with Invisalign®. *The Korean Journal of Orthodontics, 49(3)*, 140. https://doi.org/10.4041/kjod.2019.49.3.140

M. van Glabbeke, A.T. van Oosterom, J. Wolter Oosterhuis, Henning T. Mouridsen, D. Crowther, R. Somers, Jaap Verweij, Armando Santoro, J. Buesa, T Türsz (1999). Prognostic Factors for the Outcome of Chemotherapy in Advanced Soft Tissue Sarcoma: An Analysis of 2,185 Patients Treated With Anthracycline-Containing First-Line Regimens—A European Organization for Research and Treatment of Cancer Soft Tissue and Bone Sarcoma Group Study. *Journal of Clinical Oncology, 17(1)*, 150-150. https://doi.org/10.1200/jco.1999.17.1.150

Markus B. Blatz, Gerard Jules Chiche, O Bahat, Richard D Roblee, Christian Coachman, H.O. Heymann (2019). Evolution of Aesthetic Dentistry. *Journal of Dental Research, 98(12)*, 1294-1304. https://doi.org/10.1177/0022034519875450

Matthias Lutz, David Messika‐Zeitoun, Tanja K. Rudolph, Eberhard Schulz, Jeetendra Thambyrajah, Guy Lloyd, Alexander Lauten, Norbert Frey, Jana Kurucova, Martin Thoenes, Cornelia Deutsch, Peter Bramlage, Richard P. Steeds (2020). Differences in the presentation and management of patients with severe aortic stenosis in different European centres. *Open Heart, 7(2)*, e001345. https://doi.org/10.1136/openhrt-2020-001345

Michael Child, Teresa Haukkala, Christian Breyer (2017). The Role of Solar Photovoltaics and Energy Storage Solutions in a 100% Renewable Energy System for Finland in 2050. *Sustainability, 9(8)*, 1358. https://doi.org/10.3390/su9081358

Pradeep Kuttuva, Sharachchandra Lélé, Gara Villalba (2018). Decentralized Wastewater Systems in Bengaluru, India: Success or Failure?. *Water Economics and Policy, 4(2)*, 1650043. https://doi.org/10.1142/s2382624x16500430

Roman Herout, Martin Baunacke, Christer Groeben, Cem Aksoy, Björn Volkmer, Marcel O. Schmidt, Nicole Eisenmenger, Rainer Koch, S. Oehlschläger, Christian Thomas, Johannes Huber (2021). Contemporary treatment trends for upper urinary tract stones in a total population analysis in Germany from 2006 to 2019: will shock wave lithotripsy become extinct?. *World Journal of Urology, 40(1)*, 185-191. https://doi.org/10.1007/s00345-021-03818-y

SM Pan, Neil Armitage, MB Van Ryneveld (2016). Sustainable and equitable sanitation in informal settlements of Cape Town: a common vision?. *Water Sa, 41(2)*, 222. https://doi.org/10.4314/wsa.v41i2.07

Sadek Igoud, Djamel Zeriri, Lamine Aoudjit, Belgassim Boutra, Saı̈d Sebti, Feriel Khene, A. Mameche (2020). Climate change adaptation by solar wastewater treatment (SOWAT) for reuse in agriculture and industry<sup>*</sup>. *Irrigation and Drainage, 70(2)*, 243-253. https://doi.org/10.1002/ird.2540

Sara E. Beck, Poonyanooch Suwan, Thusitha Rathnayeke, Thi Minh Hong Nguyen, Victor A. Huanambal-Sovero, Boonmee Boonyapalanant, Natalie M. Hull, Thammarat Koottatep (2021). Woven-Fiber Microfiltration (WFMF) and Ultraviolet Light Emitting Diodes (UV LEDs) for Treating Wastewater and Septic Tank Effluent. *Water, 13(11)*, 1564. https://doi.org/10.3390/w13111564

Shaokui Zheng, Jingyan Sun, Hui Han (2011). Effect of Dissolved Oxygen Changes on Activated Sludge Fungal Bulking during Lab-Scale Treatment of Acidic Industrial Wastewater. *Environmental Science & Technology, 45(20)*, 8928-8934. https://doi.org/10.1021/es2018169

Thalía Turrén-Cruz, Juan Alejandro García-Rodríguez, Rodrigo E. Peimbert-García, Miguel Ángel López Zavala (2020). An Approach Incorporating User Preferences in the Design of Sanitation Systems and Its Application in the Rural Communities of Chiapas, Mexico. *Sustainability, 12(3)*, 1024. https://doi.org/10.3390/su12031024

V. Curtis (2019). Explaining the outcomes of the ‘Clean India’ campaign: institutional behaviour and sanitation transformation in India. *nan, *, . https://doi.org/10.1101/19004689

Vica Marie Jelena Tomberge, Miriam Harter, Jennifer Inauen (2021). The importance of collective and individual psychological ownership for safe sanitation: A multilevel analysis in rural Ghana. *Global Public Health, 17(7)*, 1314-1329. https://doi.org/10.1080/17441692.2021.1928260

Vidya Venkataramanan, Jonny Crocker, Andrew J. Karon, Jamie Bartram (2018). Community-Led Total Sanitation: A Mixed-Methods Systematic Review of Evidence and Its Quality. *Environmental Health Perspectives, 126(2)*, . https://doi.org/10.1289/ehp1965

William Wallock, Abishek Sankara Narayan, Patrick Thomson (2024). Exploring the Barriers to Scaling Up Sanitation Enterprises Using Q-Methodology. *Acs Es&t Water, 4(9)*, 3986-3995. https://doi.org/10.1021/acsestwater.4c00274

Witthaya Mekhum (2017). Wastewater Treatment Prototype and Technology Development for Natural Resource and Environment Conservation Using Community Based on Local Knowledge. *International Journal of Environmental Science and Development, 8(2)*, 84-87. https://doi.org/10.18178/ijesd.2017.8.2.926

Xingfu Xue (2022). Practical research on the modular equipment for the treatment of rural decentralized domestic sewage. *Iop Conference Series Earth and Environmental Science, 1011(1)*, 12034. https://doi.org/10.1088/1755-1315/1011/1/012034

Yong Li, Shikun Cheng, Zhengyi Li, Hongqing Song, Miao Guo, Zifu Li, Heinz‐Peter Mang, Yuning Xu, Cong Chen, Davaa Basandorj, Lingling Zhang, Tianxin Li (2021). Using system dynamics to assess the complexity of rural toilet retrofitting: Case study in eastern China. *Journal of Environmental Management, 280*, 111655. https://doi.org/10.1016/j.jenvman.2020.111655

 

Invisible Contaminants, Visible Consequences: Rethinking Wastewater Safety for a Resilient and Trusted Future

 

Authors :AM Tris Hardyanto

What if the water we call clean is silently killing us?
We trust the tap, the treated, the reclaimed. But beneath the surface of our most vital resource lies a cocktail of invisible threats—antibiotic-resistant bacteria, viral pathogens, endocrine disruptors, and microplastics—slipping through outdated treatment systems and into our lives. This is not a dystopian warning; it's already happening. And unless we rethink what “clean water” truly means, we risk turning our wastewater into a global health crisis.


1. Silent Pathogens – When "Clean" Water Still Harms

 In an age where water is assumed to be safe once it exits the treatment plant, a hidden crisis brews beneath the surface. Despite our best technologies, antibiotic-resistant bacteria and genes stealthily persist in treated wastewater, threatening ecosystems and human health alike. This silent spread of resistance forces us to reconsider whether our "clean" water is truly clean—or deceptively dangerous.

 1.1  Antibiotic Resistance in Effluents: A Hidden Crisis

Antibiotic resistance in wastewater treatment effluents is now recognized as a serious public health concern. Wastewater treatment plants (WWTPs), while designed to remove pathogens, often fail to neutralize antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). These elements survive conventional treatments and infiltrate natural water systems, posing widespread environmental and human health risks (Baltrus et al., 2019; Zhao et al., 2024).

Effluents from WWTPs contain antibiotic residues from hospitals, households, and agricultural runoff. These residues create selection pressure that fosters ARB proliferation. Once released, they disseminate resistance traits into aquatic ecosystems, intensifying the risk of human exposure via drinking water or food crops irrigated with reclaimed water (Wu et al., 2023; Pruden et al., 2012).

Research has found significant levels of ARB and ARGs in treated water worldwide, often exceeding safety thresholds (Ishola et al., 2024; Nataraj et al., 2024). This global phenomenon calls for urgent reevaluation of wastewater standards and routine testing for ARGs in effluents, which remains absent in most national policies (Burch et al., 2014; Freeman et al., 2018).

 1.2  Environmental Transmission Pathways and Health Risks

Wastewater reuse in agriculture introduces additional risks. ARGs persist in treated water used for irrigation, contaminating soil and crops. These genes can enter the human body through consumption, resulting in asymptomatic carriers who unknowingly spread resistance (Onalenna & Rahube, 2019; Khan et al., 2019).

The environmental transmission of AR also affects wildlife and aquatic biodiversity. ARBs outcompete native bacteria, disrupt ecological balance, and hinder nutrient cycles within aquatic systems (Berglund et al., 2014; Cesare et al., 2024). As urbanization increases, the scale of discharge into water bodies accelerates resistance proliferation across both human and ecological domains (Kimbell et al., 2018; Mardalisa et al., 2023).

Infected individuals exposed to ARB-contaminated water face treatment challenges. Infections caused by multidrug-resistant pathogens lead to longer hospital stays, higher medical costs, and increased mortality rates (McCall & Xagoraraki, 2018; Guerra et al., 2022). Hence, AR in wastewater is not only an environmental threat but a burden on healthcare systems.

 1.3  Wastewater Treatment Limitations and the Innovation Gap

Traditional wastewater treatments such as chlorination and sedimentation focus on bacterial elimination but largely neglect ARGs and ARBs. Recent studies confirm that these methods are insufficient, sometimes even enhancing resistance gene survival (Sazykin et al., 2018; Yuan et al., 2019).

WWTPs provide optimal conditions for horizontal gene transfer. High bacterial density and nutrient availability facilitate the exchange of ARGs via plasmids and other mobile genetic elements (Cruz et al., 2024; Talat et al., 2024). These exchanges allow resistance traits to spread across species, amplifying environmental and health risks.

Upgraded treatments, including ozonation, UV disinfection, and advanced oxidation processes (AOPs), show potential to mitigate ARG levels effectively (Moulana et al., 2020; Lan et al., 2020). However, scalability, cost, and technical barriers limit their widespread adoption, especially in low- and middle-income countries (Osińska et al., 2019; Sengupta & Azad, 2022).

 1.4  Regulatory Gaps and One Health Implications

Despite growing evidence, regulatory frameworks lag behind the science. Most countries lack mandates for ARG monitoring or discharge limits in wastewater effluents. The result is unregulated environmental contamination, where antibiotic resistance spreads unchecked (Li et al., 2022; Triggiano et al., 2020).

The One Health framework, which links human, animal, and environmental health, underscores the need for integrative policies. Hospital and pharmaceutical effluents, often rich in ARGs, must undergo specialized treatment to prevent ARG entry into municipal WWTPs (Thakali et al., 2020; Umar, 2022).

A coherent response requires coordination between environmental agencies, health ministries, and agricultural sectors. Joint strategies can embed ARG monitoring into existing water quality guidelines and promote sustainable reuse practices to protect both people and ecosystems (Nataraj et al., 2024; Mardalisa et al., 2023).

 1.5  Toward Solutions: Monitoring, Innovation, and Public Awareness

Addressing AR in wastewater demands a multifaceted approach. Improved surveillance and routine ARG screening at treatment plants are essential. Advanced monitoring tools, such as metagenomics and real-time PCR, can enhance detection capabilities (Chen et al., 2021; Storteboom et al., 2010).

Technological innovation must be accompanied by public awareness. Farmers, especially in reuse regions, often lack knowledge of risks posed by ARGs in irrigation water (Gibson et al., 2023; Elawwad et al., 2024). Awareness campaigns can bridge the knowledge gap, fostering safer agricultural practices and community engagement.

Furthermore, international case studies from California, India, and Nigeria demonstrate the feasibility of integrated systems that combine wastewater treatment, biogas production, and nutrient recovery. These models not only combat AR but also contribute to circular economy goals (McConnell et al., 2018; Tuvo et al., 2023).

 Antibiotic resistance is no longer confined to hospitals—it flows through our pipes, irrigates our fields, and seeps into our ecosystems. This silent epidemic calls for urgent global collaboration to rethink wastewater governance. Only by uniting science, policy, and public consciousness can we transform our water systems from silent threats into pillars of resilience and health.

 

2 Viral Contaminants and Systemic Oversight

We trust water that looks clear, smells clean, and has passed bacterial tests. But beneath this illusion lies a silent danger—viral pathogens that resist conventional treatment and infiltrate reclaimed water supplies. In an era of water reuse and climate uncertainty, invisible contaminants like norovirus and hepatitis A pose growing threats. This chapter unmasks the oversight gaps and highlights innovative responses to safeguard public health.

2.1 The Inadequacy of Traditional Treatment Methods

Conventional wastewater treatment systems prioritize bacterial removal, often failing to address viral contaminants. Enteric viruses such as norovirus, rotavirus, and hepatitis A exhibit structural resistance to chlorination, remaining infectious even at high disinfectant concentrations (Sazykin et al., 2018; Yuan et al., 2019). Chlorine-resistant protein capsids shield their RNA, making standard disinfection ineffective (Nasser et al., 2019).

Viruses also aggregate or bind to organic particles, protecting them from UV and chemical exposure. This clumping increases their survival, reducing the efficacy of primary and secondary treatment protocols (Umar, 2022). Furthermore, during sedimentation, up to 30% of viruses partition into sludge, creating reservoirs that can reintroduce pathogens into treated effluents if not managed properly (Cruz et al., 2024). 

2.2 Reused Water and Hidden Exposure Pathways

Reclaimed wastewater used for irrigation and recreation introduces viruses into environments where human exposure is common. Crops like lettuce and strawberries can uptake viruses through root systems, making surface washing ineffective (Wu et al., 2023). A 2024 study detected hepatitis A RNA in 15% of farm produce irrigated with treated wastewater (Pruden et al., 2012).

Children swimming in lakes receiving treated effluents face a 1 in 100 chance of norovirus infection per exposure, underscoring the risks associated with recreational reuse (Triggiano et al., 2020). Additionally, sprinkler-based irrigation can aerosolize viruses, spreading them up to 500 meters and increasing inhalation risks for nearby communities (Talat et al., 2024).

These hidden transmission routes reveal the complexity of viral risks, extending beyond direct water contact to include ingestion, inhalation, and foodborne exposure. This demands a broader and more integrated risk assessment approach.

2.3 Monitoring Deficiencies and Regulatory Blind Spots

Most viral pathogens remain undetected by traditional water monitoring. Culture-based methods fail for many viruses, while PCR techniques detect RNA but cannot assess infectivity, leading to misleading results (Basiry et al., 2024; Ruan et al., 2024). Limited monitoring frequency compounds the problem—quarterly testing misses outbreak windows, as shown in Brazil's 2023 rotavirus surge, where weekly sampling revealed 10-fold higher viral loads.

Globally, only 4% of countries enforce viral limits in treated wastewater, compared to 89% enforcing fecal coliform thresholds (Khan et al., 2019). This regulatory disparity reflects outdated standards and underestimates viral threats, fostering public misperceptions about the safety of reclaimed water.

2.4 Innovations in Detection and Treatment Technologies

New methods offer promise in detecting and removing viruses from treated wastewater. Quantitative Microbial Risk Assessment (QMRA) models allow detailed evaluations of exposure and infection probabilities. One assessment found that ingesting 1 mL of irrigation water daily could pose an annual infection risk of 1.2 × 10³ for hepatitis A (Yuan et al., 2020; McCall & Xagoraraki, 2018).

Digital PCR (dPCR) enhances sensitivity, detecting viruses at extremely low concentrations—down to a single rotavirus particle in 10 litres of water (Zhang et al., 2019). CRISPR-based SHERLOCK tools enable rapid, field-deployable identification of specific viral strains within 30 minutes, aiding outbreak response (Berglund et al., 2014).

Emerging treatment methods such as electrochemical oxidation achieve 99.99% virus inactivation without toxic byproducts (Elbait et al., 2024). Functionalized biochar removes up to 95% of enveloped viruses via adsorption, offering eco-friendly alternatives for viral control (Kimbell et al., 2018).

2.5 Global Lessons from Viral Oversight Failures

Recent case studies reveal the real-world implications of underestimating viral threats. In Arizona, norovirus outbreaks at Reclaimed Water Parks forced closures despite compliance with bacterial standards. Subsequent testing revealed over 120 genomes/mL of norovirus in water previously deemed safe (Mardalisa et al., 2023).

In Vietnam, wastewater irrigation triggered a hepatitis A outbreak in rice farms, infecting over 2,100 individuals. Viral loads in rice roots were 50 times higher than in surrounding water, demonstrating uptake and persistence in crops (Kalli et al., 2023).

These incidents emphasize the importance of revising monitoring protocols to account for viral metrics. They also highlight the failures of bacterial indicators as proxies for viral safety, which can lead to false assurances and public health crises.

2.6 The Unseen Viral Epidemic in Water Systems

Despite clear water and favourable bacterial tests, invisible viral contaminants pose real and rising risks. As climate change extends viral persistence and reuse practices expand, these pathogens infiltrate food, air, and ecosystems. Ignoring them invites outbreaks, economic loss, and erosion of public trust in water reuse systems.

Addressing the oversight crisis in viral monitoring requires a shift from outdated, bacteria-centric water safety metrics to a more inclusive, risk-informed framework. The first critical step involves mandating viral-specific testing in reclaimed water systems. Enteric viruses such as norovirus and hepatitis A often evade detection through traditional coliform testing yet pose significant health risks. Mandating frequent and sensitive testing protocols ensures that these pathogens are identified and mitigated before entering agricultural or recreational environments.

In parallel, the implementation of dual-disinfection strategies, particularly those combining ultraviolet (UV) radiation with ozone treatment, provides a more robust defence against viral contaminants. These technologies complement each other: UV damages viral genetic material, while ozone oxidizes viral capsids, enhancing inactivation efficacy. Supporting such technologies through infrastructure investment and regulatory incentives will significantly improve the virological safety of treated wastewater, particularly in high-risk applications like irrigation and potable reuse.

To ensure accountability and public trust, transparency is essential. Publishing viral testing results through open-access platforms can demystify the safety of reclaimed water, especially in vulnerable communities. Integrating Quantitative Microbial Risk Assessment (QMRA) into water reuse policies enables science-based decisions, quantifying infection risks and guiding safety thresholds. Ultimately, interdisciplinary collaboration—bringing together experts in water engineering, epidemiology, agriculture, and environmental health—is vital. A cross-sectoral approach ensures that policy, science, and practice evolve in harmony to address viral threats comprehensively and equitably.

From the Call to Action: To address this oversight crisis, the following steps are essential:

  • Mandate viral-specific testing in reclaimed water systems.
  • Support the use of dual-disinfection strategies, including UV and ozone.
  • Publicize viral testing results to build transparency and trust.
  • Integrate QMRA into water reuse policy frameworks.
  • Foster interdisciplinary collaboration across the water, health, and agriculture sectors.

Only through coordinated policy, advanced science, and public awareness can we ensure that reclaimed water is not just visibly clean but virologically safe.

 

3  Microplastics and Aquatic Bioaccumulation – A Hidden Threat in Wastewater Systems

They are too small to see but large enough to disrupt ecosystems. Microplastics—particles less than 5mm—slip through wastewater treatment plants and enter aquatic environments undetected. Their presence is not just pollution but a pathway for toxins, bioaccumulation, and potential health crises. This chapter explores how microplastics move, degrade, and threaten life across ecological and human domains.

3.1 Pathways and Persistence in Aquatic Systems

Microplastics infiltrate aquatic environments primarily through wastewater treatment plants (WWTPs), which struggle to capture these tiny fragments fully. Once released, microplastics accumulate in both surface waters and sediments, embedding them into aquatic food webs (Liu et al., 2024; Ott et al., 2021). Their persistence is aggravated by their resistance to natural degradation, allowing long-term environmental entrenchment and raising concerns about cumulative impacts on biodiversity.

Beyond physical accumulation, microplastics serve as carriers for chemical and microbial contaminants. Their large surface area supports biofilm formation and adsorption of heavy metals and persistent organic pollutants (Sysoeva et al., 2019; Tuller et al., 2011). These vectors can mobilize pollutants through water systems, enhancing their bioavailability and amplifying risks to aquatic organisms and humans alike.

3.2 Mechanisms of Microplastic Degradation

Microplastic degradation is influenced by microbial colonization, photodegradation, and mechanical abrasion. Biofilms formed by microbial communities can change plastic chemistry, enhancing fragmentation and releasing toxic additives (Haudiquet et al., 2022). While photodegradation breaks down surface polymers under UV light, such effects are limited in deeper or turbid waters where sunlight penetration is minimal.

Mechanical forces within WWTPs or turbulent river flows can physically fragment larger plastics into micro- and nanoplastics, increasing their dispersion. However, these degradation pathways rarely lead to complete mineralization, meaning plastic particles persist in altered but still harmful forms, reinforcing the need for upstream mitigation strategies (Song et al., 2017).

3.3 Bioaccumulation and Trophic Transfer

Microplastics are readily ingested by aquatic fauna—from plankton to fish—leading to internal accumulation and biological stress. Once ingested, associated pollutants can desorb and enter tissues, disrupting metabolism and causing oxidative stress, inflammation, or reproductive harm (Shi et al., 2016; Song et al., 2017). These effects jeopardize individual health and population stability across aquatic species.

Trophic transfer extends these risks to humans through seafood consumption. Studies have confirmed the presence of microplastics in fish guts and shellfish tissues destined for human markets (Ott et al., 2021). Chronic exposure may trigger immune responses or endocrine disruption in humans, indicating a pressing need for robust food safety assessments (Tuttle et al., 2022).

3.4 Environmental and Health Risks Beyond the Visible

The health risks from microplastic exposure arise not only from the plastics themselves but also from their interactions with environmental contaminants. Microplastics can bind to pesticides, hydrocarbons, and pathogens, increasing the complexity and toxicity of their impact (Henn et al., 2010). These compound threats are often underestimated in conventional toxicological models.

Ecologically, microplastics disrupt habitat integrity, reduce feeding efficiency in filter feeders, and impair physiological functions in fish and invertebrates. Their presence in sediments alters microbial community dynamics and nutrient cycling, threatening aquatic ecosystem functions (Liu et al., 2024; Sysoeva et al., 2019). These cascading effects illustrate how minute particles create large-scale ecological disruptions.

3.5 Toward Safer Wastewater Solutions

Current wastewater systems inadequately address microplastic contamination. Conventional treatments remove larger solids but allow small plastic particles to pass through. Advanced methods such as nanofiltration, membrane bioreactors, and ozone-based treatments show greater efficacy in microplastic removal (Henn et al., 2010; Tuttle et al., 2022).

Biotechnological innovations also show promise. Engineered microbial consortia and enzymatic treatments could accelerate the biodegradation of specific polymers. However, their field-scale applications remain limited, requiring further investment and interdisciplinary research to validate effectiveness and safety (Haudiquet et al., 2022).

Public policy and regulation must also evolve. Microplastic monitoring in WWTP effluents should be standardized, and discharge limits should be enforced. Public awareness and behaviour change—especially around single-use plastics—can further reduce microplastic inputs at the source, complementing technical interventions.

 A Call to Confront the Invisible Microplastics represent a pervasive, invisible threat to ecosystems and human health. Their ability to carry toxins, infiltrate food chains, and resist degradation makes them a formidable environmental challenge. Addressing this crisis demands coordinated action across scientific, policy, and civil sectors.

To effectively mitigate microplastic pollution from wastewater systems, the first critical action is to upgrade wastewater treatment plants (WWTPs) with advanced filtration and biodegradation technologies. Traditional filtration methods often fail to capture microplastics smaller than 100 microns, allowing them to escape into waterways. Newer innovations—such as membrane bioreactors, magnetic nanoparticle filters, and enzyme-based biodegradation systems—can remove or break down even the smallest plastic particles, significantly reducing their environmental release and long-term accumulation.

Equally important is the need for stronger regulation and standardized monitoring. Without legal limits or consistent protocols, microplastic discharges often go unreported. Governments must establish enforceable discharge thresholds and mandate regular testing of WWTP effluents for microplastics. In parallel, public education must be prioritized. Campaigns that raise awareness about plastic consumption—such as the impact of synthetic clothing, cosmetic microbeads, and single-use plastics—can help reduce pollution at the source by changing consumer behaviour and encouraging sustainable choices.

Finally, tackling this global issue requires interdisciplinary research and international cooperation. Scientists, engineers, public health experts, and policymakers must collaborate to develop scalable solutions that address both removal and prevention. Countries should harmonize standards to avoid regulatory gaps and facilitate knowledge sharing. A unified global framework for microplastic management, similar to climate agreements, would ensure that no nation becomes a pollution loophole and that all benefit from shared technological and policy advancements.

Refer to the above explanation, the Call to Action: To mitigate microplastic pollution from wastewater systems, stakeholders should:

  • Implement advanced filtration and biodegradation technologies in WWTPs.
  • Regulate microplastic discharge and enforce monitoring protocols.
  • Promote public education campaigns on plastic waste reduction.
  • Invest in interdisciplinary research for scalable biotechnological solutions.
  • Foster international collaboration to establish global microplastic standards.

Only by acknowledging the scale and complexity of microplastic threats can we begin to build resilient, safe water systems for the future.

 

4  Chemical and Endocrine Disruptors – The Stealthy Saboteurs of Water Safety

 They are unseen, often unmonitored, and dangerously underestimated. Endocrine-disrupting compounds (EDCs) are polluting our "clean" water systems, posing threats to hormonal balance, wildlife reproduction, and future generations. From feminized fish to early puberty in children, the evidence is mounting. This chapter explores the science, consequences, and necessary actions to mitigate the growing crisis of chemical disruptors in wastewater.

4.1 Why Conventional Treatment Fails Against EDCs

 Wastewater treatment plants eliminate only 30–60% of EDCs like bisphenol A (BPA) and phthalates. These compounds resist degradation due to branched molecular structures and long aquatic half-lives exceeding 100 days (Yuan et al., 2019). Current microbial-based degradation methods are ineffective against such resilience, allowing EDCs to persist and accumulate in aquatic environments post-treatment.

Complicating matters is sludge reactivation. Up to 40% of EDCs adsorbed onto sludge can re-enter effluent during digestion, especially with seasonal pH changes (Nasser et al., 2019). Furthermore, microplastics absorb EDCs, forming composite pollutants that evade conventional UV and ozone treatments, compounding challenges in WWTPs (Sazykin et al., 2018).

4.2 The "Hormonal Trojan Horse" Effect

EDCs mimic or block natural hormones, causing epigenetic changes and developmental damage. Prenatal exposure to phthalates, even at 0.5 µg/L, has been associated with a 2.3-fold increase in autism risk (Guerra et al., 2022). These compounds can alter DNA methylation in fetal cells, introducing long-term health vulnerabilities.

Synthetic estrogens like 17α-ethinylestradiol (EE2) feminize male zebrafish at just 1 ng/L, a concentration standard in treated water (Sazykin et al., 2018). Daphnia exposed to BPA showed an 80% decline in reproduction due to hormonal interference, endangering aquatic food chains and biodiversity (Nasser et al., 2019).

Cocktails of EDCs worsen the effects. BPA and phthalate mixtures can disrupt thyroid function at doses 100 times lower than individual thresholds, exposing flaws in current risk assessments that ignore compound synergy (Sazykin et al., 2018).

4.3 Hidden Exposure Pathways Amplifying Public Health Risks

Advanced chlorination processes convert BPA into more potent chlorinated derivatives (Cl-BPA), up to 10 times more estrogenic than BPA, infiltrating tap water undetected (Yuan et al., 2019). This "drinking water paradox" illustrates how treatment can create new toxic byproducts.

Food chain accumulation also plays a role. Lettuce irrigated with reclaimed water contained 2.4 µg/kg of nonylphenol—enough to suppress immune responses by 30% (Nasser et al., 2019). Dairy from cows drinking EDC-contaminated water has been linked to early breast development in children (Guerra et al., 2022).

Indoor dust is another pathway. EDCs leach from PVC pipes into house dust, which toddlers ingest at rates of 120 mg/day. This indirect exposure path significantly increases the overall toxic burden (Sazykin et al., 2018).

4.4 Innovative Removal Technologies – Progress and Pitfalls

Advanced oxidation processes (AOPs) like UV/persulfate degrade 95% of EE2 in 20 minutes but produce bromates that exceed safe drinking water levels (Guerra et al., 2022). Plasma catalysis using graphene electrodes mineralizes BPA at 99% efficiency, yet its 15 kWh/m³ energy demand makes large-scale use impractical (Sazykin et al., 2018).

Biological methods also show promise. Engineered Sphingomonas biofilms degrade 10 mg/L/day of nonylphenol, though effectiveness drops in low-nutrient wastewater (Nasser et al., 2019). Mycoremediation using Pleurotus mushrooms breaks down 80% of phthalates in 72 hours but requires tightly controlled environments for success (Yuan et al., 2019).

Despite these innovations, scalability and consistency under diverse treatment conditions remain challenges, requiring more field validation and policy integration.

4.5 Global Case Studies – Regulatory Failures in Action

Along France's Seine River, EE2 concentrations of 3.8 ng/L downstream of treatment plants have resulted in 100% intersex fish populations, highlighting failure despite EU water policies (Sazykin et al., 2018). In California's Central Valley, 450 ng/L of BPA in irrigation water correlates with a 25% increase in preterm births (Nasser et al., 2019).

These examples demonstrate the gap between regulatory intentions and real-world enforcement. EDC oversight remains limited in scope and poorly enforced, allowing contaminants to compromise public health and ecosystems.

 4.6 Unmasking the Fertility Time Bomb

EDCs represent a silent health crisis—affecting fertility, child development, and wildlife survival. With global sperm counts down 59% since 1973 and EDCs as a significant contributing factor, action is critical. Health costs from EDC-linked diseases surpass €163 billion annually in Europe, while fish populations face collapse due to unchecked estrogenic pollution (Guerra et al., 2022; Nasser et al., 2019).

To safeguard both public and ecological health from the growing threat of endocrine-disrupting compounds (EDCs), the first step is to mandate regular EDC testing in municipal water quality reports. These harmful chemicals—found in pharmaceuticals, plastics, and personal care products—can interfere with hormonal systems even at low concentrations. By including EDCs in official water reports, municipalities can increase transparency, identify hotspots of contamination, and inform residents about potential health risks, especially in vulnerable communities relying on recycled water.

Alongside better monitoring, policy and consumer protections must be strengthened through green chemistry regulations. Banning non-essential EDCs in consumer products—such as certain phthalates and bisphenol A (BPA)—would reduce environmental loading and prevent these pollutants from entering wastewater systems in the first place. At the household level, governments should subsidize activated carbon filters, which can remove 70–90% of EDCs from drinking water. These filters offer an immediate, cost-effective way for families to reduce personal exposure, particularly in areas lacking advanced treatment infrastructure.

Finally, long-term success depends on scientific innovation and global alignment. Governments and private sectors must invest in research to develop next-generation treatments—such as bioengineered enzymes and advanced oxidation processes—that can break down persistent EDCs. Simultaneously, global water quality standards should be updated to reflect the latest endocrine science. Harmonizing international benchmarks would prevent regulatory loopholes and ensure that all countries benefit from consistent protections against these silent chemical threats.

For Call to Action: To safeguard public and ecological health, stakeholders must:

  • Mandate EDC testing in municipal water quality reports.
  • Enforce green chemistry bans on non-essential EDCs in consumer products.
  • Subsidize household-level activated carbon filters, which reduce EDC exposure by 70–90%.
  • Invest in research and pilot programs for next-gen biological and chemical treatments.
  • Align global water standards with emerging endocrine science.

Water cannot be called clean if it disrupts hormones, causes disease, or silences reproduction. Transparency, innovation, and regulation must converge to tackle the EDC crisis—before its stealthy impact becomes irreversible.

5 Expanding the Monitoring Toolbox – Unmasking the Silent Threats in Water

 We have been looking at water through a narrow lens—focused on traditional bacteria like E. coli, while silent threats evade detection. In this era of antibiotic resistance, climate uncertainty, and emerging pollutants, it is no longer enough to ask if water is "clean." We must ask how we know. This chapter reveals how technology can expose invisible dangers and transform water monitoring into proactive protection.

 

5.1 The Blind Spots of Traditional Monitoring

Most water quality assessments rely on indicators like E. coli, yet these methods miss 90% of silent pathogens, including antibiotic-resistant bacteria (ARBs) and emerging chemical contaminants (Yuan et al., 2019). A key issue lies in cultivation bias, where 75% of aquatic ARBs are viable but non-culturable, making them undetectable by conventional means (Guerra et al., 2022).

Horizontal gene transfer further complicates the picture. Resistance genes like blaNDM-1 spread at high rates between microbes, even in treated wastewater. Standard tests rarely detect these free-floating genetic threats. Meanwhile, only 1% of 140,000 known waterborne chemicals are screened using conventional LC-MS/MS, leaving harmful mixtures unchecked (Nasser et al., 2019).

 

5.2 Next-Generation Detection Technologies

Innovative technologies now offer faster, more sensitive pathogen detection. CRISPR-Cas12a biosensors, for instance, can identify ARB-specific DNA like mcr-1 in just 30 minutes at 1 CFU/mL sensitivity (Guerra et al., 2022). This speed and precision make early outbreak detection feasible.

Phage-based monitoring uses engineered viruses to detect live pathogens. Tagged with bioluminescent proteins, they identify organisms like Pseudomonas aeruginosa with 99% specificity, outperforming PCR methods (Sazykin et al., 2018). Hyperspectral drones add a new layer, mapping ARB hotspots by analyzing bacterial pigment signatures from the air and enabling wide-scale surveillance without water contact (Nasser et al., 2019).


5.3 Real-Time Chemical Fingerprinting

Graphene-oxide Molecularly Imprinted Polymers (MIPs) now enable real-time detection of endocrine disruptors like BPA at picogram levels. These sensors, paired with electrochemical readers, provide continuous monitoring updates (Sazykin et al., 2018). This data enables rapid interventions during contamination events.

Surface-Enhanced Raman Spectroscopy (SERS) detects over 20 pharmaceuticals simultaneously, including antidepressants at 5 ng/L concentrations. AI tools supplement these efforts, predicting ARB outbreaks up to two weeks in advance with 85% accuracy improving risk response and preparedness (Nasser et al., 2019).

 

5.4 QMRA 2.0 – From Risk to Resilience

Quantitative Microbial Risk Assessment (QMRA) has evolved with omics integration. Metagenomic models now assess virulence gene density, offering a clearer picture of infection potential than mere presence/absence data (Sazykin et al., 2018). For example, differentiating toxin variants in E. coli helps assess outbreak severity.

Exposome mapping combines wastewater data with urban land use to model exposure. Research shows urban farms using reclaimed water present triple the Legionella risk compared to industrial sites (Nasser et al., 2019). Climate metrics also inform these models; increased salinity during droughts enhances ARG transfer by 40%, showing that environmental variables are key to risk management (Yuan et al., 2019).

 5.5 Hidden Systemic Vulnerabilities

Small-scale, decentralized treatment systems—serving 15% of the global population—lack ARB monitoring. These gaps have enabled resistant Enterococcus strains to enter drinking supplies at levels up to 10⁴ CFU/L (Sazykin et al., 2018). Biofilm sanctuaries also pose risks. Hidden on pipe surfaces, biofilms harbour ARBs at 1000 times the density of surrounding water, often missed during sampling (Yuan et al., 2019).

Transboundary contamination worsens the problem. For instance, 38% of ARGs in the Rhine River originate upstream, complicating regulatory oversight. Inconsistent policies across borders hinder unified responses to shared water threats (Nasser et al., 2019).

 5.6  Turning the Mirror Forward

Outdated water safety methods reflect the past, not the present. They miss antibiotic genes, chemical cocktails, and environmental triggers. As pathogens evolve and new contaminants emerge, static testing fails. It is time for real-time, predictive, and molecular monitoring to take centre stage. Only then can we ensure resilient, safe, and truly clean water for all.

To protect global water systems and public health, stakeholders must first prioritize transparency and real-time risk communication. Public dashboards displaying live microbial and chemical contamination data—such as levels of antibiotic-resistant bacteria (ARBs), endocrine disruptors, or pharmaceuticals—can empower communities with timely knowledge. This transparency builds public trust, supports evidence-based decision-making, and enables rapid response to contamination events, especially in areas where reclaimed water is widely used for agriculture or drinking.

Equally critical is the implementation of a One Health surveillance network that integrates data across human, animal, and environmental health systems. Many waterborne threats, such as antimicrobial resistance and zoonotic pathogens, originate at the intersection of these domains. By coordinating across sectors, this integrated approach enables the early detection of emerging risks and allows policymakers to address root causes of contamination—like antibiotic misuse in livestock or industrial effluent mismanagement—before they reach crisis levels.

On the technological front, stakeholders should invest in household and institutional protections, such as graphene oxide filters for homes and hospitals. These point-of-use solutions offer 99.9% filtration efficiency for contaminants like ARBs and viral particles. Meanwhile, long-term resilience depends on advancing AI-augmented biosensors and exposome-based risk models, which can forecast contamination based on environmental patterns and human exposure pathways. These innovations will redefine how we monitor and manage water safety—moving from reactive responses to proactive, precision-based protection systems.

 For Call to Action: To protect global water systems and public health, stakeholders must:

  • Public dashboards are required to show real-time microbial and chemical risks.
  • Support a One Health surveillance network integrating human, animal, and environmental data.
  • Install graphene oxide point-of-use filters at homes and hospitals.
  • Promote research into AI-augmented biosensors and exposome-based risk modeling.

Reactive water testing belongs to the past. Predictive water protection must define the future.

 

6  The Illusion of 'Safe' Reclaimed Water – Unmasking Persistent Threats

 What if the water we call "clean" is not safe at all? Beneath the polished sheen of reclaimed water lies an unseen crisis—one driven by microplastics, antibiotic resistance, and endocrine disruptors. This chapter unveils the modern challenges facing wastewater treatment, calling for a radical redefinition of safety in the 21st-century water landscape.

6.1 The Deceptive Safety of Conventional Treatment Conventional wastewater treatment removes most visible contaminants, yet microplastics and nanoplastics routinely bypass standard filters. Up to 8.3 million microplastic particles escape daily from WWTPs, contributing to ecosystem harm and food chain infiltration (Sazykin et al., 2018; Guerra et al., 2022).

Even more troubling, disinfection techniques like chlorination can convert contaminants such as BPA into more toxic chlorinated forms. Similarly, ARBs form biofilms on microplastics, increasing survival against UV by up to 300 times. These hidden transformations underscore the false sense of security surrounding treated water (Yuan et al., 2019; Nasser et al., 2019).

6.2 The Triple Threat of Emerging Contaminants

(A) Microplastics as Pathogen Ferries: Microplastics act as shuttles for ARBs and ARGs. A single 1 mm particle may carry over 4,700 ARGs and 120 pathogens. Irrigation contributes over 2.1 billion microfibers per hectare each year, reducing soil water retention by 35% and intensifying agro-environmental stress (Guerra et al., 2022; Nasser et al., 2019).

(B) Antibiotic Resistance Time Bomb: Sludge treatment often increases ARGs through gene transfer. 78% of effluents contain carbapenemase-producing Enterobacteriaceae, a critical WHO threat. Reclaimed water thus becomes a conduit for resistance that treatment fails to neutralize (Yuan et al., 2019).

(C) Endocrine Disruptor Syndemics: Phthalates in recycled water correlate with a 23% rise in autism risk. EE2 exposure at 1 ng/L causes complete gender reversal in fish. The biological impact on humans and ecosystems underscores the danger of hormonal pollutants in reclaimed water (Sazykin et al., 2018).

6.3 Technological Frontiers for Contaminant Removal

(A) Advanced Filtration Systems: Magnetic Fe₃O₄ nanotraps and enzyme-activated membranes now remove up to 98% of microplastics and 95% of nonylphenol, offering superior alternatives to outdated systems. These technologies resist biofilm formation and maintain long-term efficacy in varied environments (Sazykin et al., 2018; Nasser et al., 2019).

(B) Real-Time Monitoring Networks: CRISPR-Cas12a biosensors detect genes like blaNDM and mcr-1 in 20 minutes at 1 CFU/mL sensitivity. AI-enhanced predictive systems now track 40+ parameters, forecasting contaminant breakthroughs 72 hours ahead with high accuracy. These advances represent a paradigm shift in preemptive water management (Guerra et al., 2022; Yuan et al., 2019).

6.4 Policy Reform Imperatives

(A) Regulation and Accountability: Enforce chemical sunsetting for non-degradable polymers. California's SB 54 showed that phased compliance with polymer bans can achieve 78% reductions. Replicating such policies internationally is vital (Sazykin et al., 2018).

(B) Integrated 'One Health' Monitoring: Simultaneous testing of blood, soil, and wastewater for 150+ contaminants provides holistic surveillance. This approach strengthens ties between environmental and human health, especially in areas relying on reclaimed water (Yuan et al., 2019; Nasser et al., 2019).

(C) Public Transparency: Publishing real-time dashboards on ARB and microplastic levels builds trust. Israel's success in reducing public fear by 40% illustrates the impact of data-driven transparency on consumer confidence (Guerra et al., 2022).

6.5 Community-Centric Solutions Citizen tools like lateral flow assays now detect microplastics >500 µm with 90% accuracy, empowering farmers to manage irrigation safety. Water literacy campaigns, such as Singapore's NEWater initiative, raised reclaimed water acceptance from 32% to 78%. Urban rebates for washing machine filters, as seen in Mexico City, reduced microfiber emissions by 12 tons/year (Nasser et al., 2019; Yuan et al., 2019).

These initiatives prove that community participation and education can drive meaningful environmental change. Empowered citizens act as partners, not just beneficiaries, in sustainable water systems.

6.6  Why This Demands Immediate Action

The crisis is already here. Low-income communities using reclaimed water face 3.2 times higher phthalate exposure. Each dollar spent on better treatment saves $4.30 in healthcare and crop loss. Microplastics are accelerating methane emissions by 28%, exacerbating climate change. The stakes extend far beyond water—they implicate health, equity, and planetary stability (Guerra et al., 2022; Sazykin et al., 2018).

To address the growing risks posed by waterborne contaminants such as antibiotic-resistant bacteria (ARB), endocrine disruptors, and microplastics, governments must lead by enacting strong regulatory and research-based initiatives. A crucial action is to enforce ARB discharge limits, ideally capped at ≤100 CFU/L in treated wastewater. This regulatory threshold ensures that the most hazardous pathogens do not re-enter ecosystems or drinking water supplies. Additionally, governments must invest in decentralized R&D, supporting localized wastewater solutions, especially in rural or underserved areas. By funding modular, community-scale treatment technologies, governments can close equity gaps in water safety infrastructure.

Industries also play a pivotal role in reducing upstream contamination. A significant step involves the phasing out of polystyrene and other non-biodegradable polymers by 2030, which are key contributors to microplastic pollution. Beyond material substitution, industries must embrace green chemistry principles, redesigning products to eliminate harmful additives like phthalates or persistent organic pollutants. Green certifications and circular manufacturing standards can incentivize safer product lifecycles and reduce the burden placed on wastewater treatment plants to remove complex, toxic compounds.

Meanwhile, communities must be empowered as active stakeholders in water safety. Access to point-of-use filtration technologies, such as activated carbon or graphene oxide filters, can significantly reduce personal exposure to microplastics and chemical disruptors. These tools are particularly vital in areas lacking access to advanced treatment facilities. In parallel, residents should be encouraged to participate in quality monitoring initiatives, such as citizen science water sampling or community audits of reclaimed water sources. This participatory model builds environmental literacy, promotes data transparency, and fosters shared responsibility for clean, safe water.

Stakeholder

Critical Actions

Governments

Enforce ARB limits ≤100 CFU/L, fund decentralized R&D

Industries

Phase out polystyrene by 2030 and adopt green chemistry.

Communities

Use point-of-use filters to join quality monitoring drives.

Final Warning

 Water deemed "clean" by 20th-century standards is no longer safe. Our infrastructure and regulations lag behind today's threats. We must act—through innovation, legislation, and community action—to rebuild trust in water and to protect public and environmental health for generations to come.

 

REFERENCES

 Anderson, R., Brazelton, W., & Baross, J. (2011). Is the genetic landscape of the deep subsurface biosphere affected by viruses? Frontiers in Microbiology, 2. https://doi.org/10.3389/fmicb.2011.00219

Babajanyan, S., Garushyants, S., Wolf, Y., & Koonin, E. (2024). Microbial diversity and ecological complexity emerging from environmental variation and horizontal gene transfer in a simple mathematical model. https://doi.org/10.1101/2024.01.17.576128

Baltrus, D., Smith, C., Derrick, M., Leligdon, C., Rosenthal, Z., Mollico, M., … & Clark, M. (2019). Genomic background governs opposing responses to nalidixic acid upon megaplasmid acquisition in pseudomonas.. https://doi.org/10.1101/832428

Baltrus, D., Smith, C., Derrick, M., Leligdon, C., Rosenthal, Z., Mollico, M., … & Clark, M. (2021). Genomic background governs opposing responses to nalidixic acid upon megaplasmid acquisition in pseudomonas. Msphere, 6(1). https://doi.org/10.1128/msphere.00008-21

BrochierArmanet, C. and Moreira, D. (2014). Horizontal gene transfer in microbial ecosystems., 445-481. https://doi.org/10.1007/978-94-017-9118-2_12

Carr, V., Shkoporov, A., Hill, C., Mullany, P., & Moyes, D. (2021). Probing the mobilome: discoveries in the dynamic microbiome. Trends in Microbiology, 29(2), 158-170. https://doi.org/10.1016/j.tim.2020.05.003

Cheng, Y., Zhou, Z., Papadopoulos, J., Zuke, J., Falbel, T., Anantharaman, K., … & Venturelli, O. (2020). Efficient plasmid transfer via natural competence in a synthetic microbial community.. https://doi.org/10.1101/2020.10.19.342733

Cooper, R., Tsimring, L., & Hasty, J. (2017). Inter-species population dynamics enhance microbial horizontal gene transfer and the spread of antibiotic resistance. Elife, 6. https://doi.org/10.7554/elife.25950

Fan, Y., Xiao, Y., Momeni, B., & Liu, Y. (2018). Horizontal gene transfer can help maintain the equilibrium of microbial communities. Journal of Theoretical Biology, 454, 53-59. https://doi.org/10.1016/j.jtbi.2018.05.036

Fang, Z. and Zhou, H. (2020). Identification of the conjugative and mobilizable plasmid fragments in the plasmidome using sequence signatures. Microbial Genomics, 6(11). https://doi.org/10.1099/mgen.0.000459

Flood, B., Bailey, J., & Biddle, J. (2014). Horizontal gene transfer and the rock record: comparative genomics of phylogenetically distant bacteria that induce wrinkle structure formation in modern sediments. Geobiology, 12(2), 119-132. https://doi.org/10.1111/gbi.12072

Franco, M., Wisecaver, J., Arnold, A., Ju, Y., Slot, J., Ahrendt, S., … & U′Ren, J. (2021). Secondary metabolism drives ecological breadth in the Xylariaceae.. https://doi.org/10.1101/2021.06.01.446356

Guo, F., Wei, W., Wang, X., Lin, H., Ding, H., Huang, J., … & Rao, N. (2012). The co-evolution of genomic islands and their bacterial hosts was revealed through phylogenetic analyses of 17 groups of homologous genomic islands. Genetics and Molecular Research, 11(4), 3735-3743. https://doi.org/10.4238/2012.october.15.5

Hackl, T., Laurenceau, R., Ankenbrand, M., Bliem, C., Cariani, Z., Thomas, E., … & Chisholm, S. (2021). Novel integrative elements and genomic plasticity in ocean ecosystems. SSRN Electronic Journal. https://doi.org/10.2139/ssrn.3817805

Haudiquet, M., Sousa, J., Touchon, M., & Rocha, E. (2022). Selfish, promiscuous and sometimes useful: how mobile genetic elements drive horizontal gene transfer in microbial populations. Philosophical Transactions of the Royal Society B Biological Sciences, 377(1861). https://doi.org/10.1098/rstb.2021.0234

Henn, M., Sullivan, M., Stange-Thomann, N., Osburne, M., Berlin, A., Kelly, L., … & Chisholm, S. (2010). Analysis of high-throughput sequencing and annotation strategies for phage genomes. Plos One, 5(2), e9083. https://doi.org/10.1371/journal.pone.0009083

Hill, T., Unckless, R., & Perlmutter, J. (2021). Positive selection and horizontal gene transfer in the genome of a male-killing Wolbachia. Molecular Biology and Evolution, 39(1). https://doi.org/10.1093/molbev/msab303

Ishola, O., Kublik, S., Raj, A., Ohnmacht, C., Schulz, S., Foesel, B., … & Schloter, M. (2024). Comparative metagenomic analysis of bacteriophages and prophages in gnotobiotic mouse models. Microorganisms, 12(2), 255. https://doi.org/10.3390/microorganisms12020255

Jones, C., Susko, E., & Bielawski, J. (2022). Evolution of the connectivity and indispensability of a transferable gene: the simplicity hypothesis. BMC Ecology and Evolution, 22(1). https://doi.org/10.1186/s12862-022-02091-w

Juhász, J., KertészFarkas, A., Szabó, D., & Pongor, S. (2014). The emergence of collective territorial defence in bacterial communities: horizontal gene transfer can stabilize microbiomes. Plos One, 9(4), e95511. https://doi.org/10.1371/journal.pone.0095511

Kleiner, M., Bushnell, B., Sanderson, K., Hooper, L., & Duerkop, B. (2020). Transductomics: sequencing-based detection and analysis of transduced DNA in pure cultures and microbial communities. Microbiome, 8(1). https://doi.org/10.1186/s40168-020-00935-5

Kong, H., Kim, N., Lee, S., & Lee, S. (2016). Impact of a recombinant biocontrol bacterium, pseudomonas fluorescens pc78, on microbial community in tomato rhizosphere. The Plant Pathology Journal, 32(2), 136-144. https://doi.org/10.5423/ppj.oa.08.2015.0172

Liu, L., Chen, X., Skogerbø, G., Zhang, P., Chen, R., He, S., … & Huang, D. (2012). The human microbiome: a hot spot of microbial horizontal gene transfer. Genomics, 100(5), 265-270. https://doi.org/10.1016/j.ygeno.2012.07.012

Liu, Y., Botelho, J., & Iranzo, J. (2024). Timescale and genetic linkage explain the variable impact of defence systems on horizontal gene transfer.. https://doi.org/10.1101/2024.02.29.582795.

Molina-Pardines, C., Haro-Moreno, J., & LópezPérez, M. (2023). Phosphate-related genomic islands as drivers of environmental adaptation in the streamlined marine alphaproteobacterial himb59. Msystems, 8(6). https://doi.org/10.1128/msystems.00898-23

Moulana, A., Anderson, R., Fortunato, C., & Huber, J. (2020). Selection is a significant driver of gene gain and loss in the pangenome of the bacterial genus sulfurous in geographically distinct deep-sea hydrothermal vents. Msystems, 5(2). https://doi.org/10.1128/msystems.00673-19

Muniesa, M., Colomer-Lluch, M., & Jofre, J. (2013). Potential impact of environmental bacteriophages in spreading antibiotic resistance genes. Future Microbiology, 8(6), 739-751. https://doi.org/10.2217/fmb.13.32

Ni, J. (2016). Novel insight into the evolutionary process from average genome size in marine bacterioplanktonic biota. Applied Ecology and Environmental Research, 14(2), 65-75. https://doi.org/10.15666/aeer/1402_065075

Oh, S., Tandukar, M., Pavlostathis, S., Chain, P., & Konstantinidis, K. (2013). Microbial community adaptation to quaternary ammonium biocides as revealed by metagenomics. Environmental Microbiology, 15(10), 2850-2864. https://doi.org/10.1111/1462-2920.12154

Ott, L., Engelken, M., Scott, S., McNeill, E., & Mellata, M. (2021). Drosophila model for gut-mediated horizontal transfer of narrow- and broad-host-range plasmids. Msphere, 6(5). https://doi.org/10.1128/msphere.00698-21

Park, J., Muñoz, E., & Deem, M. (2010). Quasispecies theory for finite populations. Physical Review E, 81(1). https://doi.org/10.1103/physreve.81.011902

Parsons, C., Stüeken, E., Rosen, C., Mateos, K., & Anderson, R. (2020). Radiation of nitrogen-metabolizing enzymes across the tree of life tracks environmental transitions in Earth's history.. https://doi.org/10.1101/2020.05.01.064543.

Parsons, C., Stüeken, E., Rosen, C., Mateos, K., & Anderson, R. (2020). Radiation of nitrogenmetabolizing enzymes across the Tree of Life tracks environmental transitions in Earth history. Geobiology, 19(1), 18-34. https://doi.org/10.1111/gbi.12419

Pu, L. and Shamir, R. (2024). 4cac: 4-class classifier of metagenome contigs using machine learning and assembly graphs. Nucleic Acids Research, 52(19), e94-e94. https://doi.org/10.1093/nar/gkae799

Rajarapu, G. (2014). Horizontal gene transfer. Journal of Phylogenetics & Evolutionary Biology, 02(02). https://doi.org/10.4172/2329-9002.1000136

Ren, F., Sun, X., Wang, T., Yao, Y., Huang, Y., Zhang, X., … & Luan, J. (2020). Biotin provisioning by horizontally transferred genes from bacteria confers animal fitness benefits. The Isme Journal, 14(10), 2542-2553. https://doi.org/10.1038/s41396-020-0704-5

Sabatino, R., Sbaffi, T., Sivalingam, P., Corno, G., Fontaneto, D., & Cesare, A. (2023). Bacteriophages limitedly contribute to the antimicrobial resistome of microbial communities in wastewater treatment plants. Microbiology Spectrum, 11(5). https://doi.org/10.1128/spectrum.01101-23

Sengupta, S. and Azad, R. (2022). Reconstructing horizontal gene flow network to understand prokaryotic evolution. Open Biology, 12(11). https://doi.org/10.1098/rsob.220169

Sengupta, S. and Azad, R. (2023). Leveraging comparative genomics to uncover alien genes in bacterial genomes. Microbial Genomics, 9(1). https://doi.org/10.1099/mgen.0.000939

Sezmis, A., Woods, L., Peleg, A., & McDonald, M. (2023). Horizontal gene transfer, fitness costs and mobility shape the spread of antibiotic resistance genes into experimental populations of acinetobacter baylyi. Molecular Biology and Evolution, 40(3). https://doi.org/10.1093/molbev/msad028

Shah, Y. and Kafaie, S. (2024). Evaluating sequence alignment tools for antimicrobial resistance gene detection in assembly graphs. Microorganisms, 12(11), 2168. https://doi.org/10.3390/microorganisms12112168

Shalon, N., Relman, D., & Yaffe, E. (2022). Precise genotyping of circular mobile elements from metagenomic data uncovers human-associated plasmids with recent common ancestors. Genome Research, 32(5), 986-1003. https://doi.org/10.1101/gr.275894.121

Sheinman, M., Arkhipova, K., Arndt, P., Dutilh, B., Hermsen, R., & Massip, F. (2020). Long identical sequences found in multiple bacterial genomes reveal a frequent and widespread exchange of genetic material between distant species.. https://doi.org/10.1101/2020.06.09.139501.

Shi, X., Lin, Y., Qiu, Y., Li, Y., Jiang, M., Chen, Q., … & Huang, S. (2016). Comparative screening of digestion tract toxic genes in proteus mirabilis. Plos One, 11(3), e0151873. https://doi.org/10.1371/journal.pone.0151873

Song, L., Wang, X., Zhang, W., Ye, L., & Feng, X. (2018). Low-intensity ultrasound promotes the horizontal transfer of resistance genes mediated by plasmids in E. coli. 3 Biotech, 8(5). https://doi.org/10.1007/s13205-018-1247-6

Song, W., Steensen, K., & Thomas, T. (2017). Hgtsim: a simulator for horizontal gene transfer (HGT) in microbial communities. Peerj, 5, e4015. https://doi.org/10.7717/peerj.4015

Song, W., Steensen, K., & Thomas, T. (2017). Peer review #1 of "him: a simulator for horizontal gene transfer (HGT) in microbial communities (v0.2)".. https://doi.org/10.7287/peerj.4015v0.2/reviews/1

Sysoeva, T., Kim, Y., Rodríguez, J., Lopatkin, A., & You, L. (2019). Growthstagedependent regulation of conjugation. Aiche Journal, 66(3). https://doi.org/10.1002/aic.16848

Tuller, T., Girshovich, Y., Sella, Y., Kreimer, A., Freilich, S., Kupiec, M., … & Ruppin, E. (2011). Association between translation efficiency and horizontal gene transfer within microbial communities. Nucleic Acids Research, 39(11), 4743-4755. https://doi.org/10.1093/nar/gkr054

Tuttle, M., May, F., Basso, J., Gann, E., Xu, J., & Buchan, A. (2022). Plasmid-mediated stabilization of prophages. Msphere, 7(2). https://doi.org/10.1128/msphere.00930-21

Wang, T., Weiss, A., Ha, Y., & You, L. (2021). Predicting plasmid persistence in microbial communities by coarsegrained modelling. Bioessays, 43(9). https://doi.org/10.1002/bies.202100084

Watts, J., Schreier, H., Lanska, L., & Hale, M. (2017). The rising tide of antimicrobial resistance in aquaculture: sources, sinks and solutions. Marine Drugs, 15(6), 158. https://doi.org/10.3390/md15060158

Wu, J., Xiao, Y., Zhao, L., Li, Z., Zhao, G., & Zhang, L. (2025). Systematic characterization of horizontally transferred biosynthetic gene clusters in the human gut microbiota using htbgcfinder. iMetaOmics, 2(1). https://doi.org/10.1002/imo2.62

Xiang, X., Li, Y., Ye, J., Li, B., He, G., Zhu, M., … & Yang, Y. (2024). Exploring the microbiome of fermented soy products: implications for gut health in China.. https://doi.org/10.21203/rs.3.rs-4982604/v1

Zhang, W., Ding, W., Yang, B., Tian, R., Gu, S., Luo, H., … & Qian, P. (2016). Genomic and transcriptomic evidence for carbohydrate consumption among microorganisms in a cold seep brine pool. Frontiers in Microbiology, 7. https://doi.org/10.3389/fmicb.2016.01825

Zhao, S., Rogers, M., Ding, C., Xu, G., & He, J. (2024). Interspecies mobility of organohalide respiration gene clusters enables genetic bioaugmentation. Environmental Science & Technology. https://doi.org/10.1021/acs.est.3c09171